Purification and Characterization of 3-Isopropylmalate Dehydrogenase from Thiobacillus thiooxidans
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概要
- 論文の詳細を見る
3-Isopropylmalate dehydrogenase was purified to homogeneity from the acidophilic autotroph Thiobacillus thiooxidans. The native enzyme was a dimer of molecular weight 40000. The apparent K_m vvalues for 3-isopropylmalate and NAD^+ were estimated to be 0.13 mM and 8.7 mM, respectively. The optimum pH for activity was 9.0 and the optimum temperature was 65℃. The properties of the enzyme were similar to those of the Thiobacillus ferrooxidans enzyme, expect for substrate specificity. T. thiooxidans 3-isopropylmalate dehydrogenase could not utilize malate as a substrate.
- 社団法人日本生物工学会の論文
- 2000-10-25
著者
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Tano T
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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Tano Tatsuo
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Tano Tatsuo
Department Of Agricultural Chemistry Faculty Of Agriculture Okayama University
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田中 孝明
新潟大・工・機能材料
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Inagaki Kenji
Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama Uni
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KAWAGUCHI Hiroshi
Department of Orthopaedic Surgery, The University of Tokyo
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NAKAYAMA Yumi
Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The Universit
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Furutani Y
Okayama Univ. Okayama Jpn
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Inagaki Kenji
Dep. Of Bioresources Chemistry Graduate School Of Natural Sci. And Technol. Okayama Univ. 1-1-1 Tsus
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Inagaki Kenji
Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive
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Inagaki Kenji
Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ
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TANAKA HIDEHIKO
Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University
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Tanaka H
Institute Of Information Security
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Kawahara Hidehisa
Department of Biotechnology, Faculty of Engineering, Kansai University
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MATSUNAMI HIDEYUKI
Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University
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Tanaka Hidehiko
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Tanaka Hidehiko
Institute Of Information Security
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Hidehisa Kawahara
Department Of Biotechnology Faculty Of Engineering Kansai University:high Technology Research Center
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Kawaguchi Hiroshi
Department Of Chemistry Faculty Of Science Kochi University
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Tano T
Okayama Univ. Okayama Jpn
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Kawaguchi H
Department Of Orthopaedic Surgery Graduate School Of Medicine The University Of Tokyo
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Matsunami Hideyuki
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Nakayama Yumi
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Inagaki Kenji
Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive
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Inagaki K
Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive
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Kawaguchi Hiroshi
Department Of Applied Chemistry Faculty Of Engineering Utsunomiya University
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Kawaguchi Hiroshi
Department Of Orthopaedic Surgery The University Of Tokyo
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INAGAKI KENJI
Research Institute for Wakan-Yaku, Toyama Medical and Pharmaceutical University
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