Purification and Properties of an Acidic β-Glucosidase from Acidobacterium capsulatum

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概要

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• Acidobacterium capsulatum, an acidophilic, mesophilic and chemoorganotrophic bacterium, produced an inducible, acidic β-glucosidase in the cellobiose medium. The enzyme was successively purified 109 times by CM-Sepharose, Sephacryl S-200 chromatography and preparative discontinuous polyacrylamide gel electrophoresis. Polyacrylamide gel electrophoresis of the purified enzyme gave a single band at pH4.3. The enzyme had an optimum pH of 3.0 and optimum reaction temperature of 55℃, being stable from pH 1.5 to 6.0 and at temperatures from 20 to 45℃. No activity was detected above pH 6.5 or above 65℃. The molecular weight of 90,000 was estimated by gel filtration and the enzyme had an isoelectric point of 7.0. THe enzyme hydrolyzed aryl-β-glycosides and β-linked disaccharides.

• 社団法人日本生物工学会の論文
• 1991-05-25

著者

• Inagaki Kenji

  Division of Bioresources Science, Faculty of Agriculture, Okayama University

• Kishimoto Noriaki

  Mimasaka Women's Junior College

• Tano Tatsuo

  Division of Bioresources Science, Faculty of Agriculture, Okayama University

• Sugio Tsuyoshi

  Division of Bioscience, The Graduate School of Natural Science and Technology, Okayama University

• Tano T

  Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive

• Tano Tatsuo

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Tano Tatsuo

  Division Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Univers

• Furutani Y

  Okayama Univ. Okayama Jpn

• Inagaki Kenji

  Dep. Of Bioresources Chemistry Graduate School Of Natural Sci. And Technol. Okayama Univ. 1-1-1 Tsus

• Sugio Tsuyoshi

  Division Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Univers

• Sugio Tsuyoshi

  Department Of Agricultural Chemistry Faculty Of Agriculture Okayama University

• Tano T

  Okayama Univ. Okayama Jpn

• Inagaki Kenji

  Division Of Bioresources Science Faculty Of Agriculture Okayama University

• Kishimoto Noriaki

  Mimasaka Women's Junior College

• Inagaki K

  Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive

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