Existence of an Iron-Oxidizing Bacterium Acidithiobacillus ferrooxidans Resistant to Organomercurial Compounds(Environmental Biotechnology)

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Acidithiobacillus ferrooxidans MON-1 which is highly resistant to Hg^<2+> could grow in a ferrous sulfate medium (pH2.5) with 0.1μM p-chloromercuribenzoic acid (PCMB) with a lag time of 2d. In contrast, A. ferrooxidans AP19-3 which is sensitive to Hg^<2+> did not grow in the medium. Nine strains of A. ferrooxidans, including seven strains of the American Type Culture Collection grew in the medium with a lag time ranging from 5 to 12d. The resting cells of MON-1, which has NADPH-dependent mercuric reductase activity, could volatilize Hg^0 when incubated in acidic water (pH3.0) containing 0.1μM PCMB. However, the resting cells of AP19-3, which has a similar level of NADPH-dependent mercuric reductase activity compared with MON-1, did not volatilize Hg^0 from the reaction mixture with 0.1μM PCMB. The activity level of the 11 strains of A. ferrooxidans to volatilize Hg^0 from PCMB corresponded well with the level of growth inhibition by PCMB observed in the growth experiments. The resting cells of MON-1 volatilized Hg^0 from phenylmercury acetate (PMA) and methylmercury chloride (MMC) as well as PCMB. The cytosol prepared from MON-1 could volatilize Hg^0 from PCMB (0.015nmol mg^<-1> h^<-1>), PMA (0.33nmol mg^<-1> h^<-1>) in the presence of NADPH and β-mercaptoethanol.
社団法人日本生物工学会の論文
2005-06-25