Noncompetitive Inhibition by L-Cysteine and Activation by L-Glutamate of the Iron-Oxidizing Activity of a Mixotrophic Iron-Oxidizing Bacterium Strain OKM-9(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
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概要
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A mesophilic, mixotrophic iron-oxidizing bacterium strain OKM-9 uses ferrous iron as a sole source of energy and L-glutamate as a sole source of cellular carbon. Uptake of L-glutamate into OKM-9 cells is absolutely dependent on ferrous iron oxidation. Thus, the Fe^<2+>-dependent L-glutamate uptake system of strain OKM-9 is crucial for the bacterium to grow mixotrophically in iron medium with L-glutamate. The relationship between iron oxidation and L-glutamate transport activities was studied. Iron oxidase containing cytochrome a was purified 9-fold from the plasma membrane of OKM-9. A purified iron oxidase showed one rust-colored band following disc gel electrophoresis after incubation with Fe^<2+>. The Fe^<2+>-dependent L-glutamate transport system was also purified 14.5-fold from the plasma membrane using the same purification steps as for iron oxidase. Fe^<2+>-dependent L-glutamate and L-cysteine uptake activities of OKM-9 were 0.36 and 0.24 nmol/mg/min, respectively, when a concentration of 18 mM of these amino acids was used as a substrate. Both uptake activities were completely inhibited by potassium cyanide (KCN), suggesting that cytochrome a in the iron oxidase is involved in the transport process. The iron-oxidizing activity of strain OKM-9 was activated 1.7-fold by 80 mM L-glutamate. In contrast, the activity was noncompetitively inhibited by L-cysteine. The Michaelis constant of iron oxidase for Fe^<2+> was 12.6 mM and the inhibition constant for L-cysteine was 41.6 mM. A marked inhibition of iron oxidase by 50 mM L-cysteine was completely reversed by the addition of 60 mM L-glutamate. The results suggest the possibility that iron oxidase has a binding site for L-cysteine and the cysteine first bound to the iron oxidase was replaced by the added L-glutamate.
- 2004-08-25
著者
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SUGIO TUSYOSHI
Division of Science and Technology for Energy Conversion, Graduate School of Natural Science and Tec
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INOUE TAKAO
Division of Science and Technology for Energy Conversion, Graduate School of Natural Science and Tec
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KITANO YOSIKI
Faculty of Agriculture, Okayama University
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TAKEUCHI FUMIAKI
Administration Center for Environmental Science and Technology, Okayama University
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KAMIMURA KAZUO
Faculty of Agriculture, Okayama University
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Sugio Tsuyoshi
Division Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Univers
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Kamimura Kazuo
Faculty Of Agriculture Okayama University
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Kitano Yosiki
Faculty Of Agriculture Okayama University
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Sugio Tusyoshi
Division Of Science And Technology For Energy Conversion Graduate School Of Natural Science And Tech
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Takeuchi Fumiaki
Administration Center For Environmental Science And Technology Okayama University
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Inoue Takao
Division Of Morphological Analysis Department Of Functional Morphological And Regulatory Science Fac
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Inoue Takao
Division Of Science And Technology For Energy Conversion Graduate School Of Natural Science And Tech
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Kitano Yoshiki
Faculty of Agriculture, Okayama University
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