Cloning and Expression of the Thiobacillus Ferroxidans 3-Isopropylmalate Dehydrogenase Gene in Escherichia coli

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概要

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• The 3-isopropylmalate (3-IPM) dehydrogenase [EC 1.1.1.85] gene leuB of an acidophilic autotroph Thiobacillus ferrooxidans was cloced and expressed in Escherichia coli. Recombinant plasmids pTFL1 and pTEL2,carrying a 6.7-kb PstI fragment in the opposite orientation, conferred the same level of 3-IPM dehydrogenase activity on an E. coli leuB mutant. Restriction endonuclease mapping and deletion analysis indicated that 3-IPM dehydrogenase gene was on a 3.1-kb PstI-NruI fragment. The expression of the 3-IPM dehydrogenase gene of T. ferooxidans was repressed by the addition of leucine in the culture medium of E. coli carrying the plasmid pTFTL1. These results indicate that the 6.7-kb fragment contains the leu B structural gene and its regulatory region.

• 社団法人日本生物工学会の論文
• 1990-08-25

著者

• Inagaki Kenji

  Division of Bioresources Science, Faculty of Agriculture, Okayama University

• Tano Tatsuo

  Division of Bioresources Science, Faculty of Agriculture, Okayama University

• Tanaka Hidehiko

  Division of Bioresources Science, Faculty of Agriculture, Okayama University

• Sugio Tsuyoshi

  Division of Bioscience, The Graduate School of Natural Science and Technology, Okayama University

• Tano T

  Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive

• Tano Tatsuo

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Tano Tatsuo

  Division Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Univers

• KAWAGUCHI Hiroshi

  Division of Motor and Sensory System Medicine, University of Tokyo Hospital

• Furutani Y

  Okayama Univ. Okayama Jpn

• Inagaki Kenji

  Dep. Of Bioresources Chemistry Graduate School Of Natural Sci. And Technol. Okayama Univ. 1-1-1 Tsus

• Sugio Tsuyoshi

  Division Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Univers

• KUWATA KYASUYUKI

  Division of Biological Function and Genetic Resources Science, Faculty of Agriculture, Okayama Unive

• Tanaka H

  Institute Of Information Security

• Kawahara Hidehisa

  Department of Biotechnology, Faculty of Engineering, Kansai University

• Tanaka Hidehiko

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Tanaka Hidehiko

  Institute Of Information Security

• Kuwata Kyasuyuki

  Division Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Univers

• Tano T

  Okayama Univ. Okayama Jpn

• Inagaki Kenji

  Division Of Bioresources Science Faculty Of Agriculture Okayama University

• Inagaki K

  Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive

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