Transformation of the Acidophilic Heterotroph Acidiphilium facilis by Electroporation
スポンサーリンク
概要
- 論文の詳細を見る
We constructed a cloning vector for use in the acidophilic heterotroph Acidiphilium facilis. The vector pAH101 (8.8kb) was constructed from a 6.1 kb restriction fragment of the Acidiphilium plasmid pAH1 and a pUC19 carrying a β-lactamase gene. The antibiotic resistance gene was efficiently expressed in A. facilis. Several factors which influenced the transformation efficiency were optimized, resulting in a transformation efficiency of up to 3×10^3 transformants per μg of plasmid DNA at a field strength of 10 kV/cm with a 7.0 ms pulse.
- 社団法人日本農芸化学会の論文
- 1993-10-23
著者
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Inagaki Kenji
Division of Bioresources Science, Faculty of Agriculture, Okayama University
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Kishimoto Noriaki
Mimasaka Women's Junior College
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Tano Tatsuo
Division of Bioresources Science, Faculty of Agriculture, Okayama University
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Tanaka Hidehiko
Division of Bioresources Science, Faculty of Agriculture, Okayama University
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Tano T
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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Tano Tatsuo
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Tano Tatsuo
Division Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Univers
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Furutani Y
Okayama Univ. Okayama Jpn
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Inagaki Kenji
Dep. Of Bioresources Chemistry Graduate School Of Natural Sci. And Technol. Okayama Univ. 1-1-1 Tsus
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Inagaki Kenji
Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive
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Tanaka H
Institute Of Information Security
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Tomono Jun
Division of Bioresources Science, Faculty of Agriculture, Okayama University
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Tanaka Hidehiko
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Tanaka Hidehiko
Institute Of Information Security
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Tomono Jun
Division Of Bioresources Science Faculty Of Agriculture Okayama University
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Tano T
Okayama Univ. Okayama Jpn
-
Inagaki Kenji
Division Of Bioresources Science Faculty Of Agriculture Okayama University
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Kishimoto Noriaki
Mimasaka Women's Junior College
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Inagaki Kenji
Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive
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Inagaki K
Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive
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