Isolation and Characterization of Restriction Endonucleases from Acidiphilium sp. 16R and 22M
スポンサーリンク
概要
- 論文の詳細を見る
Restriction endonucleases (Asp16RI and Asp22MI) have been identified from the acidophilic bacteria Acidiphilium sp. 16R and 22M. The cleavage patterns with various DNAs show that both enzymes recognize the same sequence as the PvuI restriction endonuclease (5'-CGAT↓CG-3'), which is from Proteus vulgaris ATCC13315. Most of the catalytic properties observed for Asp16RI and Asp22MI were similar to those observed for PvuI. However, unlike PvuI both enzymes efficiently cleaved DNA in the absence of NaCl or KCl. The purification yield of Asp22MI is 60 times that of PvuI.
- 社団法人日本生物工学会の論文
- 1990-02-01
著者
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Inagaki Kenji
Division of Bioresources Science, Faculty of Agriculture, Okayama University
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Kishimoto Noriaki
Mimasaka Women's Junior College
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Tano Tatsuo
Division of Bioresources Science, Faculty of Agriculture, Okayama University
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Sugio Tsuyoshi
Division of Bioscience, The Graduate School of Natural Science and Technology, Okayama University
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Tano T
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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Tano Tatsuo
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Tano Tatsuo
Division Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Univers
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Furutani Y
Okayama Univ. Okayama Jpn
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Inagaki Kenji
Dep. Of Bioresources Chemistry Graduate School Of Natural Sci. And Technol. Okayama Univ. 1-1-1 Tsus
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Sugio Tsuyoshi
Division Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Univers
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DOU DEXIAN
Division of Biological Function and Genetic Resources Science, Faculty of Agriculture, Okayama Unive
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KITA KEIKO
Central Research Laboratories, Takara Shuzo Co. Ltd.
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HIRAOKA NOBUTSUGU
Central Research Laboratories, Takara Shuzo Co. Ltd.
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Kita Keiko
Central Research Laboratories Takara Shuzo Co. Ltd.
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Dou Dexian
Division Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Univers
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Tano T
Okayama Univ. Okayama Jpn
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Inagaki Kenji
Division Of Bioresources Science Faculty Of Agriculture Okayama University
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Hiraoka Nobutsugu
Central Research Laboratories Takara Shuzo Co. Ltd.
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Kishimoto Noriaki
Mimasaka Women's Junior College
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Inagaki K
Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive
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