Purification and Some Properties of Glutathione Reductase from Iron-oxidizing Bacterium Thiobacillus ferrooxidans
スポンサーリンク
概要
- 論文の詳細を見る
Glutathione reductase was purified from iron-grown Thiobacillus ferrooxidas AP19-3 to an electrophoretically homogeneous state. The enzyme had an apparent molecular weight of 100,000 and was composed of two identical subunits of molecular weight (M_rs, 52,000) as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A purified enzyme reduced one mole of the oxidized form of glutathione (GSSG) with one mole of NADPH to produce two moles of the reduced form of glutathione (GSH) and one mole of NADP^+. The glutathione reductase was most active at pH 6.5 and 40℃, and had an isoelectric point at 5.1. The Michaelis constants of glutathione reductase for GSSG, NADPH, and NADH were 300, 26, and 125μM, respectively.
- 社団法人日本農芸化学会の論文
- 1995-08-23
著者
-
Tano Tatsuo
Division of Bioresources Science, Faculty of Agriculture, Okayama University
-
Sugio Tsuyoshi
Division of Bioscience, The Graduate School of Natural Science and Technology, Okayama University
-
Tano T
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
-
Tano Tatsuo
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
-
Tano Tatsuo
Division Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Univers
-
Sugio Tsuyoshi
Division Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Univers
-
Sugio Tsuyoshi
Department Of Agricultural Chemistry Faculty Of Agriculture Okayama University
-
TANAKA TSUYOSHI
Division of Earth and Environmental Sciences, Nagoya University
-
Matsugi Shinji
Division of Biological Function and Genetic Resources Science, Faculty of Agriculture, Okayama Unive
-
Fujita Masahiro
Division of Biological Function and Genetic Resources Science, Faculty of Agriculture, Okayama Unive
-
Matsugi Shinji
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
-
Fujita Masahiro
Division Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Univers
-
Tanaka Tsuyoshi
Division Of Biotechnology And Life Science Institute Of Engineering Tokyo University Of Agriculture And Technology
関連論文
- 精密ろ過膜とオンライン乳酸コントローラーを備えた培養システムを用いたPropionibacterium shermaniiによるビフィズス菌特異的増殖促進物質(BGS)の生産(生物工学論文賞)
- Growth-inhibition of hiochi bacteria in namazake (raw sake) by bacteriocins from lactic acid bacteria(BREWING AND FOOD TECHNOLOGY)
- Restriction Endonuclease Aor13HI from Acidiphilium organovorum 13H, a New Isoschizomer of BspMII : Purification and Characterization
- Volatilization of Metal Mercury from Organomercurials by Highly Mercury-Resistant Acidithiobacillus ferrooxidans MON-1
- 1J10-2 酒母工程における乳酸菌バクテリオシンの火落菌に対する殺菌効果(食品科学,食品工学,一般講演)
- 2K10-4 生分解性ポリエステル多孔質体の力学特性の評価(生体医用工学,人工臓器/動物細胞工学,動物組織培養,一般講演)
- バイオマスプラスチック製濾過膜の開発と応用 (特集 新しいバイオマス利活用技術)
- 1Mp22 米タンパク質由来ペプチドのヒト病原菌に対する抗菌作用とその機構の解明(食品科学・食品工学,一般講演)
- 1Mp21 酒造工程における乳酸菌由来バクテリオシンの火落菌に対する殺菌作用(食品科学・食品工学,一般講演)
- 非対称型デプスフィルターによる非圧縮性粒子懸濁液の精密濾過
- 食品微生物を用いた有用生理活性物質の生産
- 乳酸菌バクテリオシンを用いた清酒の火落ち防止技術の開発
- ポリ乳酸の多孔質化・複合化による機能性材料の開発(循環型社会を支えるラクテートインダストリーの新たな研究潮流)
- 1Gp07 ヒラタケによって脱リグニン処理した稲わらを用いたエタノールの生産(培養工学,一般講演)
- 1Gp08 酵素を用いた多収米の液化糖化の最適化とエタノール生産の低コスト化(培養工学,一般講演)
- 2Ip07 清酒製造工程における乳酸菌バクテリオシンの火落菌に対する増殖阻害作用(食品科学・食品工学,一般講演)
- 2Ip08 歯周病菌に対する米由来抗菌タンパク質の検索および分離同定(食品科学・食品工学,一般講演)
- 2Ip09 抗菌活性測定法の高感度化とそれを用いた植物由来抗菌タンパク質の検索(食品科学・食品工学,一般講演)
- 2Ip16 プロテアーゼ阻害成分を含む米タンパク質の品質向上用食品素材としての評価(食品科学・食品工学,一般講演)
- 2F14-2 多収米および精米残渣の酵素糖化とそれらの発酵原料としての利用(培養工学,センサー・計測工学・ロボット工学,プロセス工学,一般講演)
- 2F14-3 担子菌によって処理した稲わらの組成変化とその発酵原料としての有用性の評価(培養工学,センサー・計測工学・ロボット工学,プロセス工学,一般講演)
- 1H11-3 歯周病菌に対する米由来抗菌タンパク質の検索とそれらの性質(食品科学・食品工学,一般講演)
- 3S1AM5 ポリ乳酸の多孔質化・複合化による機能性材料の開発(循環型社会を支えるラクテートインダストリーの新たな研究潮流,シンポジウム)
- 1H10-2 プロテアーゼ阻害成分を含む米タンパク質の機能性食品素材としての利用(食品科学・食品工学,一般講演)
- 1H10-1 水産練り製品中の腐敗細菌の同定とそれらの乳酸菌バクテリオシンによる増殖抑制(食品科学・食品工学,一般講演)
- 834 有機溶媒を含む反応系における芳香族化合物の酵素酸化重合
- 1F16-2 火落菌に対して抗菌活性を示すバクテリオシンの生産とその性質(食品科学・食品工学,一般講演)
- 3D11-4 麹汁培地を用いて生産した乳酸菌バクテリオシンの火落ち菌に対する抗菌作用(発酵生理学・発酵工学・培養工学,一般講演)
- Effect of steam explosion pretreatment on treatment with Pleurotus ostreatus for the enzymatic hydrolysis of rice straw(ENVIRONMENTAL BIOTECHNOLOGY)
- 2D16-2 担子菌によって脱リグニン処理した稲わらの発酵原料としての利用(発酵生理学・発酵工学・培養工学,一般講演)
- Application of a Recombinant Plasmid Expressing Desensitized 3-Deoxy-D-Arabinoheptulosonate-7-Phosphate Synthase to the Breeding of L-Phenylalanine Producers
- Cloning and Characterization of Genes Responsible for m-Fluoro-D,L-phenylalanine Resistance in Brevibacterium lactofermentum(Microbiology & Fermentation Industry)
- Genetic Breeding of L-Tyrosine Producer from Brevibacterium lactofermentum(Microbiology & Fermentation Industry)
- S123 ビフィズス菌とプロピオン酸菌間の協調作用の解析とその同時菌体生産への応用
- 974 膜型混合バイオリタクターを用いたビフィズス菌の効果的生産
- Production of Bifidogenic Growth Stimulator by Co-cultivation of Propionibacterium shermanii and Lactobacillus Strain Using Lactose as a Carbon Source
- Production of Extracellular Bifidogenic Growth Stimulator(BGS) from Propionibacterium shermanii Using a Bioreactor System with a Microfiltration Module and an On-line Controller for Lactic Acid Concentration(BIOCHEMICAL ENGINEERING)
- Production of Extracellular Bifidogenic Growth Stimulator by Anaerobic and Aerobic Cultivations of Several Propionibacterial Strains(BIOCHEMICAL ENGINEERING)
- Ferrous Iron Production Mediated by Tetrathionate Hydrolase in Tetrathionate-, Sulfur-, and Iron-Grown Acidithiobacillus ferrooxidans ATCC 23270 Cells
- Isolation and Characterization of Acidithiobacillus ferrooxidans Strain D3-2 Active in Copper Bioleaching from a Copper Mine in Chile
- Reduction of Hg2+ with Reduced Mammalian Cytochrome c by Cytochrome c Oxidase Purified from a Mercury-Resistant Acidithiobacillus ferrooxidans Strain, MON-1
- Increase in Fe2+-Producing Activity during Growth of Acidithiobacillus ferrooxidans ATCC23270 on Sulfur
- Existence of aa_3-Type Ubiquinol Oxidase as a Terminal Oxidase in Sulfite Oxidation of Acidithiobacillus thiooxidans
- Growth Inhibition by Tungsten in the Sulfur-Oxidizing Bacterium Acidithiobacillus thiooxidans
- Existence of an Iron-Oxidizing Bacterium Acidithiobacillus ferrooxidans Resistant to Organomercurial Compounds(Environmental Biotechnology)
- Existence of a Tungsten-Binding Protein in Acidithiobacillus ferrooxidans AP19-3(ENVIRONMENTAL BIOTECHNOLOGY)
- Volatilization of Mercury under Acidic Conditions from Mercury-polluted Soil by a Mercury-resistant Acidithiobacillus ferrooxidans SUG 2-2(Microbiology & Fermentation Technology)
- Cytochrome c Oxidase Purified from a Mercury-Resistant Strain of Acidithiobacillus ferrooxidans Volatilizes Mercury
- Purification and Some Properties of Sulfur Reductase from the Iron-Oxidizing Bacterium Thiobacillus ferrooxidans NASF-1
- 3P-1167 Porphyromonas gingivalisに対して抗菌活性を有する新規米由来タンパク質の検索と同定(4b食品科学,食品工学,一般講演,醸造・食品工学,伝統の技と先端科学技術の融合)
- 3P-1166 米タンパク質由来CHペプチドの口腔細菌に対する抗菌作用とその機構の解明(4b食品科学,食品工学,一般講演,醸造・食品工学,伝統の技と先端科学技術の融合)
- Inhibition of Iron-oxidizing Activity by Bisulfite Ion in Thiobacillus ferrooxidans
- Cloning and Expression of the Thiobacillus Ferroxidans 3-Isopropylmalate Dehydrogenase Gene in Escherichia coli
- Growth Inhibition of Acidiphilium Species by Organic Acids Contained in Yeast Extract
- Isolation and Characterization of Restriction Endonucleases from Acidiphilium sp. 16R and 22M
- Isolation and Some Properties of an Iron-Oxidizing Bacterium Thiobacillus ferrooxidans Resistant to Bisulfite Ion
- Ferrous-iron-dependent Uptake of L-Glutamate by a Mesophilic, Mixotrophic Iron-oxidizing Bacterium Strain OKM-9(Microbiology & Fermentation Technology)
- Purification and Characterization of Sulfur Reductase from a Moderately Thermophilic Bacterical Strain, TI-1,that Oxidizes Iron
- Production of Hydrogen Sulfide by a Moderately Thermophilic Iron-Oxiding Bacterium Strain TI-1
- Production of Hydrogen Sulfide from Tetrathionate by the Iron-Oxidizing Bacterium Thiobacillus ferrooxidans NASF-1
- Isolation and Some Properties of a Mesophilic and Mixotrophic Iron-oxidizing Bacterium, OKM-9
- Isolation of an Acremonium sp. Capable of Liquefying Cross-Linked Poly(γ-Glutamic Acid) Hydrogels and the Fungal Enzyme Involved in the Disruption of γ-Ray Irradiation-Mediated Cross-Linking(MISCELLANEOUS)
- FE-P6 Growth-inhibitory activity of antimicrobial peptides from rice against oral pathogenic bacteria and clarification of their action mechanism(Section IX Food Engineering)
- Purification and Some Properties of Glutathione Reductase from Iron-oxidizing Bacterium Thiobacillus ferrooxidans
- Purification and Some Properties of NADH-Dependent Sulfite Reductase from Escherichia coli Harboring Plasmid pTHS1,Which Has the S-Adenosyl-L-Methionine : Uroporphyrinogen III Methyltransferase Gene of Thiobacillus ferrooxidans
- A DNA Region That Complements on Escherichia coli cysG Mutation in Thiobacillus ferrooxidans
- NADH-dependent Sulfite Reductase Activity in the Periplasmic Space of Thiobacillus ferrooxidans
- Inhibition of Sulfur Use by Sulfite Ion in Thiobacillus ferrooxidans(Microbiology & Fermentation Industry)
- Purificaion and Some Properties of a Hydrogen Sulfide-binding Protein That Is Involved in Sulfur Oxidation of Thiobacillus ferrooxidans(Microbiology & Fermentation Industry)
- Effects of Polyamine on the Growth of Acidiphilium facilis 24R under Acidic Conditions(Microbiology & Fermentation Industry)
- The Mechanism of Copper Leaching by Intact Cells of Thiobacillus ferrooxidans(Microbiology & Fermentation Industry)
- The Regulation of Sulfur Use by Ferrous Ion in Thiobacillus ferrooxidans(Microbiology & Fermentation Industry)
- Purification and Characterization of Glycerol 3-Phosphate Dehydrogenase from Two Types of Acidiphilium sp.(Biological Chemistry)
- Effect of Co^ on the GSH Uptake and Release by Thiobacillus ferrooxidans(Microbiology & Fermentation Industry)
- Mechanism of Tetravalent Manganese Reduction with Elemental Sulfur by Thiobacillus ferrooxidans(Microbiology & Fermentation Industry)
- Purification and Characterization of 3-Isopropylmalate Dehydrogenase from Thiobacillus thiooxidans
- Transformation of the Acidophilic Heterotroph Acidiphilium facilis by Electroporation
- Purification and Properties of an Acidic β-Glucosidase from Acidobacterium capsulatum
- Identification of Phoslactomycin E as a Metabolite Inducing Hyphal Morphological Abnormalities in Aspergillus fumigatus IFO 5840
- Amplification of Vacuole-targeting Fungicidal Activity of Antibacterial Antibiotic Polymyxin B by Allicin, an Allyl Sulfur Compound from Garlic
- 非対称型デプスフィルターによる細菌懸濁液の精密濾過
- Evaluation of Fungal Pretreatments for Enzymatic Saccharification of Rice Straw
- A Combination of Unnatural Phosphatidyl Acceptor and Tandem Electrospray Ionization Mass Spectrometry for Tracing Phospholipase D Activity
- Ferrous Iron Production Mediated by Tetrathionate Hydrolase in Tetrathionate-, Sulfur-, and Iron-Grown Acidithiobacillus ferrooxidans ATCC 23270 Cells
- Increase in Fe^-Producing Activity during Growth of Acidithiobacillus ferrooxidans ATCC23270 on Sulfur
- Reduction of Hg^ with Reduced Mammalian Cytochrome c by Cytochrome c Oxidase Purified from a Mercury-Resistant Acidithiobacillus ferrooxidans Strain, MON-1
- Isolation and Characterization of Acidithiobacillus ferrooxidans Strain D3-2 Active in Copper Bioleaching from a Copper Mine in Chile
- Volatilization of Metal Mercury from Organomercurials by Highly Mercury-Resistant Acidithiobacillus ferrooxidans MON-1
- Sulfite Oxidation Catalyzed by aa_3-Type Cytochrome c Oxidase in Acidithiobacillus ferrooxidans
- 非対称型デプスフィルターによる細菌懸濁液の精密濾過
- Poly(L-lactic acid) Microfiltration Membrane Formation via Thermally Induced Phase Separation with Drying
- 2Ep19 米由来ペプチドによるチロシナーゼ活性の阻害とメラニン形成の抑制(タンパク質工学/核酸工学/ペプチド工学,一般講演)
- 2Jp05 歯周病菌に対して抗菌活性を発揮する米由来タンパク質の精製と同定(食品科学・食品工学,一般講演)
- 2Ep18 米由来ペプチドのプロテアーゼ阻害活性と抗菌活性に及ぼす塩基性アミノ酸の寄与(タンパク質工学/核酸工学/ペプチド工学,一般講演)
- 2Ep17 米タンパク質由来CHペプチドの歯周病菌に対する抗菌作用機構の解明(タンパク質工学/核酸工学/ペプチド工学,一般講演)
- 2Jp04 米由来抗菌ペプチドの口腔細菌のバイオフィルム形成に対する阻害効果(食品科学・食品工学,一般講演)
- Antimycin A-induced cell death depends on AIF translocation through NO production and PARP activation and is not involved in ROS generation, cytochrome c release and caspase-3 activation in HL-60 cells
- Enhancement effect of N-methyl-N"-dodecylguanidine on the vacuole-targeting fungicidal activity of amphotericin B against the pathogenic fungus Candida albicans
- The vacuole-targeting fungicidal activity of amphotericin B against the pathogenic fungus Candida albicans and its enhancement by allicin
- Dependence of vacuole disruption and independence of potassium ion efflux in fungicidal activity induced by combination of amphotericin B and allicin against Saccharomyces cerevisiae