Application of a Recombinant Plasmid Expressing Desensitized 3-Deoxy-D-Arabinoheptulosonate-7-Phosphate Synthase to the Breeding of L-Phenylalanine Producers

概要

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A recombinant plasmid containing a desensitized 3-deoxy-D-arabinoheptulosonate-7-phosphate (DAHP) synthase gene was used for transformation of four strains of Brevibacterium lactofermentum : AJ12036 (a wild strain), no. 29 (a tyrosine auxotroph), MF358 (a phenylalanine analogue resistant mutant), and no. 123 (a phenylalanine analogue resistant and tyrosine auxotrophic mutant). The transformants exhibited improved productivities of aromatic amino acids as follows : (i) the introduction of the plasmid converted AJ12036 to a producer of both L-phenylalanine and L-tyrosine, (ii) transformants of no. 29 produced L-phenylalanine even in a medium with excess L-tyrosine, (iii) those of MF358 produced larger amounts of L-phenylalanine and L-tyrosine than the host strain, (iv) those of no. 123 produced larger amounts of L-phenylalanine than the host strain and the production did not decrease markedly even in a medium with excess L-tyrosine. The gene dosage effect of desensitized DAHP synthase on the production of L-phenylalanine and related compounds is discussed on the basis of these results.
社団法人日本生物工学会の論文
1991-09-25