Purification and Some Properties of a Tetrathionate Decomposing Enzyme from Thiobacillus thiooxidans
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概要
- 論文の詳細を見る
A tetrathionate-decomposing enzyme that catalyzes the decomposition of tetrathionate into thiosulfate and sulfate was purified to homogeneity from tetrathionate-grown Thiobacillus thiooxidans. The enzyme had an apparent molecular weight of 104,000, and Was composed of two identical subunits (MW 58,000)as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme had an isoelectric point at 9.6 and was most active at pH 3.0-3.5 and 40℃. Enzyme activity was increased approximately 100-fold in the presence of 400 mM sulfate ion. The Michaelis constant of this enzyme for tetrathionate in the presence of 20, 50, and 200 mM of sulfate ion was 2.4 mM. Mercuric and ferric ions completely inhibited the enzyme activity at 1 mM. Though cupric ion up to 0.01 mM markedly stimulated the activity in the presence of 20 mM sulfate ion, a higher concentration (1 mM) rather strongly inhibited the activity. Ethylenediaminetetraacetic acid (EDTA) strongly inhibited the activity, but this inhibiton was completely restored by cupric ion.
- 社団法人日本農芸化学会の論文
- 1996-02-23
著者
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Nagasawa Toru
Department of Biomolecular Science, Gifu University
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Tano Tatsuo
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Tano Tatsuo
Department Of Agricultural Chemistry Faculty Of Agriculture Okayama University
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Tano Tatsuo
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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Sugio Tsuyoshi
Department of Botany and Microbiology, Division of Bioscience, Graduate School of Natural Science an
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Sugio Tsuyoshi
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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Sugio Tsuyoshi
Department Of Agricultural Chemistry Faculty Of Agriculture Okayama University
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KITAGUCHI Hajime
Department of Biological Function and Genetic Resources Science, Faculty of Agriculture, Okayama Uni
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HARADA Mitsuru
Department of Biological Function and Genetic Resources Science, Faculty of Agriculture, Okayama Uni
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Nagasawa Toru
Department Of Agricultural Chemistry Kyoto University
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Kitaguchi Hajime
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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Harada M
Miyazaki Univ. Miyazaki Jpn
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Nagasawa Toru
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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