Synthesis of D-Cysteine from a Racemate of 3-Chloroalanine by Phenylhydrazine-treated Cells of Pseudomonas putida CR 1-1

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概要

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• 3-Chloro-D-alanine chloride-lyase, which occurs in the cells of Pseudomonas putida CR 1-1, catalyzes the β-replacement reaction of 3-chloro-D-alanine in the presence of a high concentration of sodium hydrosulfide to form D-cysteine. Using the β-replacement reaction, the synthesis of D-cysteine from a racemate of 3-chloroalanine by resting cells was investigated. In order to depress the synthesis of L-cysteine from 3-chloro-L-alanine and the enzymatic degradation of 3-chloro-L-alanine, treatment of the cells with 5mM phenylhydrazine was utilized. Under the optimal conditions, 94% of the added 3-chloro-D-alanine could be converted to D-cysteine and 81% of the added 3-chloro-L-alanine was recovered.

• 社団法人 日本農芸化学会の論文

著者

• Nagasawa Toru

  Department of Biomolecular Science, Gifu University

• YAMADA Hideaki

  Department of Agricultural Chemistry, Kyoto University

• Yamada H

  Department Of Agricultural Chemistry Facutly Of Agriculture Kyoto University

• TANI Yoshiki

  Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University

• Nagasawa Toru

  Department Of Agricultural Chemistry Kyoto University

• HOSONO Hidekazu

  Department of Agricultural Chemistry, Kyoto University

• YAMANO Hironori

  Department of Agricultural Chemistry, Kyoto University

• OHKISHI Haruyuki

  Central Research Laboratory, Mitsubishi Chemical Industries

• Tani Yoshiki

  Department Of Agricultural Chemistry College Of Agriculture Kyoto University

• Yamada Hideaki

  Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University

• NAGASAWA Toru

  Department of Agricultural Chemistry, Kyoto University

• OHKISHI Haruyuki

  Central Research Laboratories, Mitsubishi Chemical Industries

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