Production of 6-Hydroxynicotinic Acid from Nicotinic Acid by Resting Cells of Pseudomonas fluorescens TN5
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概要
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Resting cells of Pseudomonas fluorescens TN5 catalyze the hydroxylation at the 6-position of nicotinic acid to produce 6-hydroxynicotinic acid. We optimized the reaction and culture conditions for the production of 6-hydroxynicotinic acid from nicotinic acid by using P. fluorescens TN5 resting cells. The addition of molybdenum and iron ions and nicotinic acid as an inducer to the culture medium significantly enhanced the formation of the hydroxylation enzyme. The supply of oxygen was important for the enhancement of hydroxylation activity. Under the optimum conditions, 98.7% of the added 1.4M nicotinic acid was converted to 6-hydroxynicotinic acid, and the highest yield was 191g of 6-hydroxynicotinic acid per liter of reaction mixture containing 7.43g dry weight of cells in 45 h at 35℃
- 社団法人日本農芸化学会の論文
- 1994-04-23
著者
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Yamane Tsuneo
Laboraotory Of Bioreaction Engineering Department Of Food Science And Technology Faculty Of Agricult
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Yamane Tsuneo
Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur
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Nagasawa Toru
Department Of Agricultural Chemistry Kyoto University
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Yamane Tsuneo
Department Of Applied Biological Sciences Faculty Of Agriculture Nagoya University
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Yamane Tsuneo
Laboratory Of Molecular Biotechnology Graduate School Of Bio- And Agro-sciences Nagoya University
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HURH BYUNGSERK
Department of Food Science and Technology, Faculty of Agriculture, Nagoya University
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Hurh Byungserk
Department Of Food Science And Technology Nagoya University
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