Increasing Productivity by Removing Choline in Conversion of Phosphatidylcholine to Phosphatidylserine by Phospholipase D

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概要

• 論文の詳細を見る

• The conversion of phosphatidylcholine (PC) to phosphatidylserine (PS) was examined in the presence of L-serine (3.4 M, solubility of serine) using two phospholipase D (PLD) preparations of Streptomyces origin. A biphasic reaction was carried out at 30℃ using an aqueous phase containing both PLD and serine and a solvent (ethyl acetate or diethyl ether) phase containing PC. With an increase in the PC concentration from 17.8 mM to 106.8 mM, the conversion decreased from 85% to 36% in 30 min. The effect of choline inhibition was investigated in two ways-by the addition of choline chloride at low PC concentrations and by removing choline enzymatically or physically during the progress of the reactin at high PC concentrations. The addition of various concentrations of choline chloride decreased the reaction rate, conversion and selectivity. The unreacted PC decreased from 36% to 17.6% and further to 1.6% by the addition of choline oxidase and a mixture of choline oxidase and catalase, respectively. There were no decreases in either the selectivity or the final PS content for the ten repeated batches examined when choline released was washed out from the PLD gels immobilized in Octyl-Sepharose CL-4B after each run.

• 社団法人日本生物工学会の論文
• 1992-05-25

著者

• YAMANE TSUNEO

  Laboratory of Molecular Biotechnology, Division of Molecular and Cellular Mechanisms, graduate Schoo

• Juneja L

  太陽化学

• Juneja Lekh

  (present Address)central Research Laboratories Taiyo Kagaku Co. Ltd.

• Yamane Tsuneo

  Laboraotory Of Bioreaction Engineering Department Of Food Science And Technology Faculty Of Agricult

• Yamane Tsuneo

  Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur

• Shimizu Shoichi

  Laboraotory Of Bioreaction Engineering Department Of Food Science And Technology Faculty Of Agricult

• Shimizu S

  Professor Emeritus Nagoya University

• Shiimizu Susumu

  Department Of Food Science And Technology Faculty Of Agriculture Nagoya University

• TANIGUCHI ERI

  Laboraotory of Bioreaction Engineering, Department of Food Science and Technology, Faculty of Agricu

• Shimizu Shoichi

  Daiichi Pharmaceutical Co. Ltd.

• Taniguchi Eri

  Laboraotory Of Bioreaction Engineering Department Of Food Science And Technology Faculty Of Agricult

• Yamane Tsuneo

  Laboratory Of Molecular Biotechnology Graduate School Of Bio- And Agro-sciences Nagoya University

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