High Speed Polymerase Chain Reaction in Constant Flow

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概要

• 論文の詳細を見る

• A new simple reactor of the tubing type was developed for polymerase chain reaction (PCR). A thin Teflon capillary tube was used as a tubing reactor in which the reaction mixture of PCR was driven by a pump at a constant flow rate. The sample was treated with three successive thermal stages for denaturation, annealing, and elongation of DNA and primers as a function of the position in the tube. The amplification yield was about a half of that obtained by a commercial thermocycler. Moreover, the total reaction time from 12 to 18 min, which was one-tenth of the time generally required by conventional thermocyclers using metal blocks, assured substantial amplification of a DNA fragment. In addition, this reactor could be also used for rapid cycle-sequences. This new device will be easily incorporated into automated and rapid DNA analysis systems for DNA sequencing.

• 社団法人日本農芸化学会の論文
• 1994-02-23

著者

• Nakano Hideo

  Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya Universit

• YAMANE TSUNEO

  Laboratory of Molecular Biotechnology, Division of Molecular and Cellular Mechanisms, graduate Schoo

• Yohda M

  Tokyo Univ. Agriculture And Technol. Tokyo

• Yohda M

  Inst. Physical And Chemical Res. (riken) Saitama Jpn

• Yamane Tsuneo

  Laboraotory Of Bioreaction Engineering Department Of Food Science And Technology Faculty Of Agricult

• Yamane Tsuneo

  Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur

• Nagamune T

  Chemical Engineering Laboratory Rlken (the Institute Of Physical And Chemical Research)

• ENDO ISAO

  Biochemical Systems Laboratory, The Institute of Physical and Chemical Research(RIKEN)

• Miyashita Yukiko

  Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology

• Matsuda Koji

  Laboratory Of Molecular Biotechnology Department Of Applied Biological Sciences School Of Agricultur

• Endo I

  Inst. Physical And Chemical Res.(riken) Saitama

• Endo I

  Inst. Physical And Chemical Res. (riken) Saitama Jpn

• Endo Isao

  Chemical Engineering Laboratory The Institute Of Physical And Chemical Research

• Matsuda K

  Waseda Univ. Tokyo Jpn

• Yohda Masafumi

  Chemical Engineering Laboratory, RlKEN (The Institute of Physical and Chemical Research)

• Nagamune Teruyuki

  Chemical Engineering Laboratory, RlKEN (The Institute of Physical and Chemical Research)

• Nakano Hideo

  Laboratory Of Molecular Biotechnology Graduate School Of Bioagricultural Sciences Nagoya University

• Nagamune Teruyuki

  Chemical Engineering Laboratory The Institute Of Physical And Chemical Research

• Yamane Tsuneo

  Laboratory Of Molecular Biotechnology Graduate School Of Bio- And Agro-sciences Nagoya University

• Yoshida Mamiko

  Precision Systems Science Co. Ltd.

• Nakano Hideo

  Laboratory Of Molecular Biotechnology Department Of Bioengineering Sciences Graduate School Of Bioag

• Nagamune Teruyuki

  Chemical Engineering Laboratory Rlken (the Institute Of Physical And Chemical Research)

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