Rapid Multiplex Single Nucleotide Polymorphism Genotyping Based on Single Base Extension Reactions and Color-Coded Beads
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概要
- 論文の詳細を見る
A Single nucleotide polymorphism (SNP) typing method using color-coded beads is promising because it is easy to use and inexpensive. However, the present protocols are not suitable for clinical and diagnostic applications because they need centrifugation for bead-washing. Here, we developed a simplified protocol without a bead-washing procedure that enables SNP typing of PCR amplified fragments in only 30 min.
- 2002-10-25
著者
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Yohda Masafumi
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
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Yohda Masafumi
Department Of Biotechnology And Life Science Graduate School Of Engineering Tokyo University Of Agri
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小原 賢信
日立製作所
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Miyashita Yukiko
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology
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小原 賢信
文部科学省科学技術・学術政策局政策課 安全・安心科学技術企画室
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Kohara Yoshinobu
Hitachi Ltd. Central Research Laboratory:department Of Biotechnology And Life Science Tokyo Universi
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Kohara Yoshinobu
Department Of Chemistry And Biotechnology Faculty Of Engineering The University Of Tokyo
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OKANO KAZUNORI
Hitachi, Ltd., Central Research Laboratory
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KAMBARA HIDEKI
Hitachi, Ltd., Central Research Laboratory
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FUJIMURA NAOKO
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
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Okano K
Hitachi Ltd. Central Research Laboratory
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Fujimura Naoko
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology:hitachi
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Kambara Hideki
Hitachi Ltd. Central Research Laboratory
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Kambara Hideki
Hitachi Ltd
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