Development of a Novel Method for Operating Magnetic Particles, Magtration Technology, and Its Use for Automating Nucleic Acid Purification
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概要
- 論文の詳細を見る
Magnetic particles are useful for simple and efficient nucleic acid extraction. To achieve fully automated nucleic acid extraction and purification using magnetic particles, a new method for operating magnetic particles, Magtration Technology, was developed. In this method, magnetic separation is performed in a specially designed disposable tip. This enables high recovery of magnetic particles with high reproducibility. The features of this technology are(i)a simple mechanism for process control and(ii)flexible software to enable adaptation to commercially available reagents. Automated instruments based on Magtration Technology were developed and used for nucleic acid extraction. Total DNA, total RNA and plasmids were purified by Magtration Technology at an efficiency comparable to that of manual methods.
- 社団法人日本生物工学会の論文
- 2001-05-25
著者
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Yohda Masafumi
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
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TAJIMA HIDEJI
Precision System Science Co., Ltd.
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Tajima Hideji
Precision Systems Science Co. Ltd.
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Yohda M
Tokyo Univ. Agriculture And Technol. Tokyo Jpn
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Yohda Masafumi
Department Of Biotechnology And Life Science Graduate School Of Engineering Tokyo University Of Agri
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MATSUNAGA Tadashi
Department of Pediatric Surgery, Chiba University School of Medicine
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IKEDA KATSUNORI
Tsuruga Institute of Biotechnology, Toyobo Co. Ltd.
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KAWAKAMI BUNSEI
Tsuruga Institute of Biotechnology, Toyobo Co. Ltd.
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Ikeda Katsunori
Tsuruga Institute Of Biotechnology Toyobo Co.ltd.
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Ikeda Katsunori
Tsuruga Institute Of Biotechnology Toyobo Co. Ltd.
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OBATA KIMIMICHI
Precision System Science Co.Ltd.
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SEGAWA OSAMU
Precision System Science Co.Ltd.
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YAKABE MITSURU
Precision System Science Co.Ltd.
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ISHIDA YOSHIKAZU
Tsuruga Institute of Biotechnology, Toyobo Co.Ltd.
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KUROITA TOSHIHIRO
Tsuruga Institute of Biotechnology, Toyobo Co.Ltd.
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KAWAMURA YOSHIHISA
Tsuruga Institute of Biotechnology, Toyobo Co.Ltd.
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Matsunaga Tadashi
Department Of Applid Chemistry For Resources Tokyo University Of Agriculture And Technology
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Kawakami Bunsei
Tsuruga Enzyme Plant Toyobo Co. Ltd.
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Kawakami Bunsei
Tsuruga Institute Of Biotechnology Toyobo Co.ltd.
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Ishida Yoshikazu
Tsuruga Institute Of Biotechnology Toyobo Co.ltd.
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Kuroita Toshihiro
Tsuruga Institute Of Biotechnology Toyobo Co. Ltd
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Kuroita Toshihiro
Tsuruga Institute Of Biotechnology Toyobo Co.ltd.
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Kawamura Yoshihisa
Tsuruga Institute Of Biotechnology Toyobo Co. Ltd.
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Kawamura Yoshihisa
Tsuruga Institute Of Biotechnology Toyobo Co.ltd.
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Matsunaga Tadashi
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology
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