Nucleotide Sequence of the Gene Coding for the BanIII DNA Methyltransferase in Bacillus aneurinolyticus(Microbiology & Fermentation Industry)

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概要

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• The gene coding for the ATCGAT specific BanIII DNA methyltransferase (M-BanIII) of Bacillus aneurinolyticus was cloned and its nucleotides sequenced. The coding region was assigned on the nucleotide sequence on the basis of the N-terminal amino acid sequence and molecular weight of the enzyme. The M-BanIII gene coded for a protein of 580 amino acid residues (MW 66,344). Comparison with other methylases indicated that the M-BanIII sequence contained a segment of tetra-amino acids, NPPY, characteristic of N6-adenine methylases. In addition, some homologous regions were found in the sequences of type II adenine methylases PaeR7I(CTCGAG), TaqI(TCGA) and PstI(CTGCAG), containing TCGA within the recognition sequences.

• 社団法人日本農芸化学会の論文

著者

• KAWAKAMI BUNSEI

  Tsuruga Institute of Biotechnology, Toyobo Co. Ltd.

• OKA MASANORI

  Tsuruga Institute of Biotechnology, Toyobo Co. Ltd.

• Sasaki Akiko

  Tsuruga Enzyme Plant Toyobo Co. Ltd.

• Oka Masanori

  Tsuruga Enzyme Plant Toyobo Co. Ltd.

• MAEKAWA Yoshihiko

  Tsuruga Enzyme Plant, Toyobo Co., Ltd.

• Maekawa Yoshihiko

  Tsuruga Enzyme Plant Toyobo Co. Ltd.

• Kawakami Bunsei

  Tsuruga Enzyme Plant Toyobo Co. Ltd.

関連論文

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• Cloning, Nucleotide Sequence, and Expression in Escherichia coli of DNA Polymerase Gene (polA) from Thermus thermophilus HB8
• Nucleotide Sequence of the Gene Coding for the BanIII DNA Methyltransferase in Bacillus aneurinolyticus(Microbiology & Fermentation Industry)
• Host-Dependent Activation of IS1203v Excision in Shiga Toxin-Producing Escherichia coli(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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• Cloning and Nucleotide Sequences of the AccI Restriction-Modification Genes in Acinetobacter calcoaceticus(Microbiology & Fermentation Industry)
• Cloning and Expression in Escherichia coli of the BanI and BanIII Restriction-Modification Systems from Bacillus aneurinolyticus

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