Cloning and Expression of the BamHI Restriction-Modification System in Bacillus subtilis
スポンサーリンク
概要
- 論文の詳細を見る
The genes of the GGATCC-specific BamHI restriction-modification system of Bacillus amyloliquefaciens H have been cloned and expressed in Bacillus subtilis MT-2. B. subtilis MT-2 carrying the recombinant plasmid (pBamHIRM22) produced about 10-fold more BamHI restriction endonuclease and BamHI methylase than B. amyloliquefaciens H did. B. subtilis MT-2 (pBamHIRM22) restricted unmodified phage. Restriction and modification genes were stably maintained in B. subtilis MT-2 on one plasmid and the produced BamHI endonuclease remained stable even in the stationary phase of the culture, BamHI endonuclease from B. subtilis MT-2 (pBamHIRM22) had the same molecular weight and N-terminal amino acid sequence as that from B. amyloliquefaciens H.
- 社団法人日本生物工学会の論文
- 1990-10-25
著者
-
IMANAKA Tadayuki
Department of Synthetic and Biological Chemistry, Graduate School of Engineering, Kyoto University
-
Imanaka Tadayuki
Department Of Applied Biotechnology Faculty Of Engineering Osaka University
-
Imanaka Tadayuki
Department Of Fermentation Technology Faculty Of Engineering Osaka University
-
KAWAKAMI BUNSEI
Tsuruga Institute of Biotechnology, Toyobo Co. Ltd.
-
MAEKAWA Yoshihiko
Tsuruga Enzyme Plant, Toyobo Co., Ltd.
-
Maekawa Yoshihiko
Tsuruga Enzyme Plant Toyobo Co. Ltd.
-
KATSURAGI NOBUHIRO
Tsuruga Enzyme Plant, Toyobo Co., Ltd.,
-
Kawakami Bunsei
Tsuruga Enzyme Plant Toyobo Co. Ltd.
-
Katsuragi Nobuhiro
Tsuruga Enzyme Plant Toyobo Co. Ltd.
-
IMANAKA TADAYUKI
Department of Fermentation Technology Faculty of Engineering, Osaka University
関連論文
- Bacterial Alginate Lyase : Enzymology, Genetics and Application
- Cellular Toxicity of Cadmium Ions and Their Detoxification by Heavy Metal-Specific Plant Peptides, Phytochelatins, Expressed in Mammalian Cells^1
- Utilization of Immobilized Archaeal Chaperonin for Enzyme Stabilization
- Enhanced Signal Transduction by a Directly Fused Protein of Interleukin-6 and Its Receptor
- Unique Nucleoid Structure during Cell Division of Thermococcus kodakaraensis KOD1
- Alteration of Product Specificity of Cyclodextrin Glucanotransferase from Thermococcus sp.B1001 by Site-Directed Mutagenesis
- Effect of Polyamines on Histone-Induced DNA Compaction of Hyperthermophilic Archaea
- Highly Branched Oligosaccharides Produced by the Transglycosylation Reaction of Neopullulanase
- Gene Cloning and Sequence Analysis of Cobyric Acid Synthase and Cobalamin (5'-phosphate) Synthase from Hyperthermophilic Archaeon Pyrococcus sp. KOD1
- Biophysical Analysis of Heat-Induced Structural Maturation of Glutamate Dehydrogenase from a Hyperthermophilic Archaeon (ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Mutational Effects on O^6-Methylguanine-DNA Methyltransferase from Hyperthermophile : Contribution of Ion-Pair Network to Protein Thermostability
- Contribution of Protein-Surface Ion Pairs of a Hyperthermophilic Protein on Thermal and Thermodynamic Stability
- Genetic, Enzymatic, and Structural Analyses of Phenylalanyl-tRNA Synthetase from Thermococcus kodakaraensis KOD1
- Biophysical Effect of Amino Acids on the Prevention of Protein Aggregation
- 超好熱菌由来タンパク質のさまざまな変性条件下での構造安定性
- Acceptor Specificity of 4-α-Glucanotransferase from Pyrococcus kodakaraensis KOD1, and Synthesis of Cycloamylose
- Characterization of FtsZ Homolog from Hyperthermophilic Archaeon Pyrococcus kodakaraensis KOD1
- Characterization and Application to Hot Start PCR of Neutralizing Monoclonal Antibodies against KOD DNA Polymerase
- Gene Analysis and Enzymatic Properties of Thermostable β-Glycosidase from Pyrococcus kodakaraensis KODI
- Crystallographic Studies on a Family B DNA Polymerase from Hyperthermophilic Archaeon Pyrococcus kodakaraensis Strain KOD1^1
- Phylogenetic Analysis and Effect of Heat on Conformational Change of Ferredoxin from Hyperthermophilic Archaeon Pyrococcus sp.KOD1
- Cloning and Expression of the 4-α-Glucanotransferase Gene from the Hyperthermophilic Archaeon Pyrococcus sp. KOD1,and Characterization of the Enzyme
- Cloning and Expression of the α-Amylase Gene from the Hyperthermophilic Archaeon Pyrococcus sp. KOD1,and Characterization of the Enzyme
- Production of Bacterial Alginate-Specific Lyase by Recombinant Bacillus subtilis
- Crystallization and Preliminary X-Ray Diffraction Studies of Bacterial Alginate Lyase
- Construction and Characterization of N-Terminally Truncated DNA Polymerase from Thermus thermophilus
- Gene Cloning and Characterization of Aldehyde Dehydrogenase from a Petroleum-Degrading Bacterium, Strain HD-1
- Development of a Novel Method for Operating Magnetic Particles, Magtration Technology, and Its Use for Automating Nucleic Acid Purification
- Characterization of Petroleum-Degrading Bacteria from Oil-Contaminated Sites in Vietnam
- Equilibrium and Kinetic Stability of a Hyperthermophilic Protein, O^6-Methylguanine-DNA Methyltransferase under Various Extreme Conditions
- Rapid Method for Detection and Detoxification of Heavy Metal Ions in Water Environments Using Phytochelatin
- A Simple Method for Determination of Substrate Specificity of Alginate Lyases
- Purification and Characterization of α-Glucan Phosphorylase from Bacillus stearothermophilus
- Production and Some Properties of a Dextrin with a Narrow Size Distribution by the Cyclization Reaction of Branching Enzyme
- Expression of Recombinant Antibody against Cancer-Specific Carbohydrate
- COMPLETE GENOME ANALYSIS AND TARGETED GENE DISRUPTION IN THE HYPERTHERMOPHILIC ARCHAEON, THERMOCOCCUS KODAKARAENSIS(Life under Extreme Environments, International Symposium on Origins of Life and Astrobiology)
- Gene Cloning and Characterization of Fructose-1,6-Bisphosphate Aldolase from the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1
- Display of Heterologous Gene Products on the Escherichia coli Cell Surface as Fusion Proteins with Flagellin
- Gentisate 1,2-dioxygenase from Xanthobacter polyaromaticivorans 127W.
- Isolation and Characterization of Xanthobacter polyaromaticivorans sp. nov. 127W That Degrades Polycyclic and Heterocyclic Aromatic Compounds under Extremely Low Oxygen Conditions
- Characterization of a Small Metalloprotease from Streptomyces caespitosus with High Specificity to Aromatic Residues
- Nucleotide Sequence of the Gene Coding for the BanIII DNA Methyltransferase in Bacillus aneurinolyticus(Microbiology & Fermentation Industry)
- Production and Characterization of Biosurfactants from Bacillus licheniformis F2.2(Microbiology & Fermentation Technology)
- Highly Thermostable Neutral Protease from Bacillus stearothemophilus
- Effect of Growth Temperature and Growth Phase on the Lipid Composition of the Archaeal Membrane from Thermococcus kodakaraensis
- Diversity in Size and Alkaliphily of Thermostable α-Amylase-Pullulanases (AapT) Produced by Recombinant Escherichia coli, Bacillus subtilis and the Wild-Type Bacillus sp.
- Rational Design for Stabilization and Optimum pH Shift of Serine Protease AprN
- Nucleotide Sequence and Possible Functional Mechanism of the Transcriptional Activator Gene (preL) for Neutral Protease from Lactobacillus sp.
- Nucleotide Sequence of the Neutral Protease Gene (nprL) from Lactobacillus sp. and Characterization of the Enzyme
- Purification and Characterization of a New Metal Protease Which Hydrolyzes the Cyclic Decapeptide, Gramicidin S
- Cloning and Nucleotide Sequence of the Regulatory Gene, degM, for Minor Serine Protease in Baciilus subtilis
- Role of the Pre-Pro-Region of Neutral Protease in Secretion in Bacillus subtilis
- Isolation and Characterization of Psychrotrophs from Subterranean Environments
- High-Level Production of Erythritol by Strain 618A-01 Isolated from Pollen
- Isolation of Bacterial Strans Colonizable in Mosquito Larval Guts as Novel Host Cells for Mosquito Control
- Gene Cloning of an Alcohol Dehydrogenase from Thermophilic Alkane-Degrading Bacillus thermoleovorans B23
- Identification of the Gene Encoding Esterase, a Homolog of Hormone-Sensitive Lipase, from an Oil-Degrading Bacterium, Strain HD-1
- Gentisate 1,2-Dioxygenase from Xanthobacter polyaromaticivorans 127W
- The Concept of the α-Amylase Family : Structural Similarity and Common Catalytic Mechanism
- Cloning and Expression of the BamHI Restriction-Modification System in Bacillus subtilis
- Effect of Growth Temperature and Growth Phase on the Lipid Composition of the Archaeal Membrane from Thermococcus kodakaraensis
- Identification of the Phr-dependent heat shock regulon in the hyperthermophilic archaeon, Thermococcus kodakaraensis
- Continuous Productin of Panose by Immobilized Neopullulanase
- Construction of Inducible Secretion Vectors and Their Application for the Secretion of Foreign Extracellular and Intracellular Proteins in Bacillus subtilis
- Gene Cloning and Characterization of Recombinant Ribose Phosphate Pyrophosphokinase from a Hyperthermophilic Archaeon
- 656 Cloning and Analysis of 16 S-rRNA gene and Transcriptional Factor TF IID gene from Hyperthermophilic Archaeum strain KOD 1
- Gene Cloning and Function Analysis of Replication Factor C from Thermococcus kodakaraensis KOD1(Biochemistry & Molecular Biology)
- Gene Cloning and Polymerase Chain Reaction with Proliferating Cell Nuclear Antigen from Thermococcus kodakaraensis KOD1(Biochemistry & Molecular Biology)
- Isolation of a New Surfactin Producer Bacillus pumilus A-1,and Cloning and Nucleotide Sequence of the Regulator Gene , psf-1
- Cloning and Nucleotide Sequences of the AccI Restriction-Modification Genes in Acinetobacter calcoaceticus(Microbiology & Fermentation Industry)
- Protein Engineering of Penicillinase as Affinity Ligands for Bioprocessing
- Isolation and Characterization of Long-Chain-Alkane Degrading Bacillus thermoleovorans from Deep Subterranean Petroleum Reservoirs
- Family B DNA polymerase from a hyperthermophilic archaeon Pyrobaculum calidifontis : Cloning, characterization and PCR application(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Modeling of Cephalosporin C Production and Its Application to Fed-batch Culture
- Regulaton of Cephalosporin C Produciton by Endogeneous Methionine in Cephalosporium acremonium
- Morphological Differenctitaion in Relation to Cephalosporin C Synthesis by Cephalosporium acremonium
- Effect of Glucose and Methionine Consumption Rates on Cephalosporin C Production by Cephalosporium acremonium
- Isolation and Application of Extremophiles.
- Analysis of a Negative Regulator, SoxR, for the Arthrobacter Sarosine Oxidase Gene
- Cloning and Sequencing of the Sarcosine Oxidase Gene from Arthrobacter sp. TE1826
- Highly Conservative Sequence in the Carboxyl Terminus of Sarcosine Oxidase is Important for Substrate Binding
- Cloning and Nucleotide Sequence of the Urea Amidolyase Gene from Candida utilis
- Gene Cloning and Characterization of Thermostable Peptidyl Prolyl cis-trans Isomerase (PPIase) from Bacillus stearothermophilus SIC1
- Cloning and Expression in Escherichia coli of the BanI and BanIII Restriction-Modification Systems from Bacillus aneurinolyticus
- Isolation of a New Mixotrophic Bacterium Which Can Fix CO_2 and Assimilate Aliphatic and Aromatic Hydrocarbons Anaerobically
- Biological Threshold Values of Procaryotic Gene Expression Which Is Controlled by the DNA Inverted Repeat Sequence and the mRNA Secondary Structure
- Effect of Amino Acid Substitutions at the Active Center on the Activity of Bacillus stearothermophilus Alcohol Dehydrogenase
- Tumor Suppressive Monoclonal Antibody Belonging to the V_H 7183 Family Directed to the Oncodevelopmental Carbohydrate Antigen on Human Hepatocellular Carcinoma
- Isolatino and Characterization of Oxygen Sensitive Mutants of Escherichia coli
- Binding ability of chitinase onto cellulose : an atomic force microscopy study
- Cloning and Nucleotide Sequence of the Gene Coding for a Subunit of the Respiratory NADH Dehydrogenase from Bacillus stearothermophilus
- Effect of Cultivation Temperatures on Thermophilic and Mesophilic Enzyme Gene Expression in Bacillus subtilis
- Characteristics of Thermostable Pullulanase from Bacillus stearothermophilus and the Nucleotide Sequence of the Gene
- A New Way of Stabilizing Recombinant Plasmids
- Adaptation Strategy of Thermophiles toward Hyperthermophily and Their Molecular Bases
- Molecular bases of thermophily in hyperthermophiles
- Cellular Toxicity of Cadmium Ions and Their Detoxification by Heavy Metal-Specific Plant Peptides, Phytochelatins, Expressed in Mammalian Cells.