Gene Cloning and Characterization of Aldehyde Dehydrogenase from a Petroleum-Degrading Bacterium, Strain HD-1

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概要

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• The hd-ald gene encoding aldehyde dehydrogenase(hd-ALDH)from an mixotrophic petroleum-degrading bacterium, strain HD-1 was cloned and sequenced. hd-ALDH(506 amino acids)is a member of the NAD^+-dependent aldehyde dehydrogenase group. The hd-ald gene was expressed in Escherichia coli, and the recombinant enzyme was purified and characterized biochemically and enzymatically. The molecular weight of the enzyme was estimated to be 55,000 by SDS-PAGE, and 224,000 by gel filtration chromatography, suggesting that it acts as a tetramer. The CD spectrum suggests that the helical content of the enzyme is 10%. hd-ALDH was active on various aliphatic aldehyde substrates. The K_m values of the enzyme were 6.4 μM for acetaldehyde, 4.2 μM for hexanal, 2.8 μM for octanal, and 0.84 μM for decanal, whereas the k_<cat> values for these substrates were nearly equal(51-64 min^<-1>). These results indicate that hd-ALDH acts preferentially on long-chain aliphatic aldehydes.

• 1999-07-25

著者

• HIRANO Shin-ichi

  Department of Applied Chemistry, Graduate School of Engineering, Nagoya University

• Hirano S

  Division Of Nanomaterials Science Ecotopia Science Institute Nagoya University

• KANAYA Shigenori

  Department of Material and Life Science, Osaka University

• 森川 正章

  阪大院・工・物生

• IMANAKA Tadayuki

  Department of Synthetic and Biological Chemistry, Graduate School of Engineering, Kyoto University

• Imanaka Tadayuki

  Department Of Applied Biotechnology Faculty Of Engineering Osaka University

• Hirano Shi-ichi

  Department Of Applied Chemistry Graduate School Of Engineering Nagoya University

• AMADA KEI

  Department of Material and Life Scinece, Graduate Shool of Engineering, Osaka University

• HARUKI MITSURU

  Department of Material and Life Scinece, Graduate Shool of Engineering, Osaka University

• MORIKAWA MASAAKI

  Department of Material and Life Scinece, Graduate Shool of Engineering, Osaka University

• Hirano Shin‐ichi

  Division Of Nanomaterials Science Ecotopia Science Institute Nagoya University

• Hirano Shin-ichi

  Central Research Laboratories Mercian Corp.

• Hirano Shin-ichi

  Department Of Applied Chemistry Graduate School Of Engineering Nagoya University

• Amada Kei

  Department Of Material And Life Science Graduate School Of Engineering Osaka University

• Amada Kei

  Department Of Life Environment And Materials Science Faculty Of Engineering Fukuoka Institute Of Tec

• OKIBE NAOKO

  Department of Material and Life Science, Graduate School of Engineering, Osaka University

• Okibe Naoko

  Department Of Material And Life Science Graduate School Of Engineering Osaka University

• Imanaka T

  Dep. Of Biotechnology Coll. Of Life Sciences Ritsumeikan Univ.

• Hirano S

  Central Research Laboratories Mercian Corp.

• Hirano Shin-ichi

  Environmental Research Center Mercian Cleantec Corporation

• Morikawa Masaaki

  Department Of Biotechnology Department Of Material & Life Science Graduate School Of Engineering

• HARUKI Mitsuru

  Department of Material and Life Science, Graduate School of Engineering, Osaka University

• Kanaya Shigenori

  Department of Material and Life Science, Graduate School of Engineering, Osaka University

• Hirano Shin-ichi

  Department of Material and Life Science, Graduate School of Engineering, Osaka University

• HARUKI Mitsuru

  Department of Chemical Biology and Applied Chemistry, College of Engineering, Nihon University

• OKIBE Naoko

  Department of Earth Resources Engineering, Kyushu University

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