Gene Cloning, Overproduction, and Characterization of Thermolabile Alkaline Phosphatase from a Psychrotrophic Bacterium
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概要
- 論文の詳細を見る
The gene encoding alkaline phosphatase from the psychrotrophic bacterium Shewanella sp. SIB1 was cloned, sequenced, and overexpressed in Escherichia coli. The recombinant protein was purified and its enzymatic properties were compared with those of E. coli alkaline phosphatase (APase), which shows an amino acid sequence identity of 37%. The optimum temperature of SIB1 APase was 50 °C, lower than that of E. coli APase by 30 °C. The specific activity of SIB1 APase at 50 °C was 3.1 fold higher than that of E. coli APase at 80 °C. SIB1 APase lost activity with a half-life of 3.9 min at 70 °C, whereas E. coli APase lost activity with a half-life of >6 h even at 80 °C. Thus SIB1 APase is well adapted to low temperatures. Comparison of the amino acid sequences of SIB1 and E. coli APases suggests that decreases in electrostatic interactions and number of disulfide bonds are responsible for the cold-adaptation of SIB1 APase.
- 社団法人 日本農芸化学会の論文
- 2005-02-23
著者
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森川 正章
大阪大学大学院工学研究科物質・生命工学専攻:北海道大学大学院地球環境科学研究院環境生物科学部門
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TAKANO Kazufumi
Department of Material and Life Science, Osaka University
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KANAYA Shigenori
Department of Material and Life Science, Osaka University
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SUZUKI Yutaka
Department of Radiology, School of Medicine, Tokai University
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森川 正章
阪大院・工・物生
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Miki Masao
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Fukui
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Mizutani Yoichi
Department of Urology, Kyoto Prefectural University of Medicine
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TSUJI TADAO
Department of Internal Medicine, Saitama Municipal Hospital
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Tsuji Tadao
Department Of Material And Life Science Graduate School Of Engineering Osaka University
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Tsuji Tadao
Department Of Gastroenterology Saitama Municipal Hospital
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HARUKI MITSURU
Department of Material and Life Scinece, Graduate Shool of Engineering, Osaka University
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MORIKAWA MASAAKI
Department of Material and Life Scinece, Graduate Shool of Engineering, Osaka University
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Takano Kazufumi
Department Of Material And Life Science Graduate School Of Engineering Osaka University
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Suzuki Yutaka
Department Of Internal Medicine Tokai University School Of Medicine
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Okibe Naoko
Department Of Material And Life Science Graduate School Of Engineering Osaka University
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Mizutani Yoichi
Department Of Material And Life Science Graduate School Of Engineering Osaka University
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OHTANI Naoto
Department of Material and Life Science, Graduate School of Engineering, Osaka University
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Morikawa Masaaki
Department Of Biotechnology Department Of Material & Life Science Graduate School Of Engineering
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Morikawa Masaaki
Department Of Material And Life Science Osaka University
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Suzuki Yutaka
Department Of Cardiology And Radiology School Of Medicine Tokyo University
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Takano Kazufumi
Department Of Material And Life Science Osaka University
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HARUKI Mitsuru
Department of Material and Life Science, Graduate School of Engineering, Osaka University
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Kanaya Shigenori
Department of Material and Life Science, Graduate School of Engineering, Osaka University
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HARUKI Mitsuru
Department of Chemical Biology and Applied Chemistry, College of Engineering, Nihon University
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SUZUKI Yutaka
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
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