Promotion of Large Protein Crystal Growth with Stirring Solution
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概要
- 論文の詳細を見る
We propose a protein crystal growth with stirring the solution, which is entirely different approach compared with the conventional method. The novel method, which we call the Floating And Stirring Technique (FAST), enables both growing crystals on an insoluble and very dense liquid without contacting the vessel and stirring the protein solution mildly. Using this method together with slow cooling, we obtained a 3.0 mm-long lysozyme crystal in 20 days from a seed crystal. This is larger than the 2.0 mm-long crystal grown without stirring. Stirring the protein solution accelerates the growth of the protein crystal and prevents additional spontaneous nucleation.
- 2002-10-01
著者
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TAKANO Kazufumi
Department of Material and Life Science, Osaka University
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Takano Kazufumi
Department Of Material And Life Science Graduate School Of Engineering Osaka University
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Yoshimura Masashi
Department Of Applied Biological Chemistry The University Of Tokyo
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Mori Yusuke
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Adachi Hiroaki
Department Of Biological Science Faculty Of Science Hiroshima University
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Sasaki Takatomo
Department Of Electrical Electronic And Information Engineering Osaka University
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ADACHI Hiroaki
Department of Electrical Engineering and Venture Business Laboratory, Osaka University
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SASAKI Takatomo
Department of Electrical Engineering and Venture Business Laboratory, Osaka University
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