Protein Crystal Growth Using Laser-Processed Seed Crystals
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概要
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We demonstrate the use of crystals processed by a pulsed UV laser as seeds for protein crystallization. A hen-egg-white lysozyme crystal, which was successfully laser-processed without causing significant damage, was seeded and grown larger than its original size. Polarized light microscopy and X-ray diffraction analysis showed that the crystal grown by seeding was a single crystal of suitable quality for X-ray crystallography. The results support the effectiveness of UV-laser processing for protein crystals. Furthermore, this seeding technique, which we call laser-cut seeding, will be useful for the production of a seed of suitable size and shape in macroseeding, for the elimination of a damaged section in a growing crystal, and for removing useless crystals from polycrystals.
- 2005-05-15
著者
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Matsumura Hiroyoshi
SOSHO Project (Crystal Design Project), Osaka University
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Inoue Tsuyoshi
SOSHO Project (Crystal Design Project), Osaka University
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Koga Yuichi
Department Of Material And Life Science Graduate School Of Engineering Osaka University
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Takeuchi Kengo
Department Of Laboratory Medicine Research Hospital The Institute Of Medical Science The University
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Takano Kazufumi
Department Of Material And Life Science Graduate School Of Engineering Osaka University
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Doi Masaaki
Core Technology Center Nikon Corporation
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Kitano Hiroshi
Core Technology Center Nikon Corporation
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Mori Yusuke
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Adachi Hiroaki
Department Of Biological Science Faculty Of Science Hiroshima University
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Sasaki Takatomo
Department Of Electrical Electronic And Information Engineering Osaka University
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Kanaya Shigenori
Department of Material and Life Science, Graduate School of Engineering, Osaka University
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Murakami Satoshi
SOSHO Inc., Osaka 541-0053, Japan
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Takano Kazufumi
Department of Material and Life Science, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
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Koga Yuichi
Department of Material and Life Science, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
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Takeuchi Kengo
Department of Material and Life Science, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
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