Laser Irradiated Growth of Protein Crystal

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概要

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• We succeeded in the first ever generation of protein crystals by laser irradiation. We call this process Laser Irradiated Growth Technique (LIGHT). Effective crystallization was confirmed by applying an intense femtosecond laser. The crystallization period was dramatically shortened by LIGHT. In addition, protein crystals were obtained by LIGHT from normally uncrystallized conditions. These results indicate that intense femtosecond laser irradiation generates crystal nuclei; protein crystals can then be grown from the nuclei that act as seeds in a supersaturated solution. The nuclei formation is possible primarily due to nonlinear nucleation processes of an intense femtosecond laser with a peak intensity of over a gigawatt (GW).

• 2003-07-15

著者

• HOSOKAWA Youichiroh

  Department of Allied Physics,Osaka University

• MASUHARA Hiroshi

  Department of Allied Physics,Osaka University

• Kai Yasushi

  Department Of Applied Chemistry Faculty Of Engineering Osaka University

• Tsunaka Yasuo

  Department Of Material And Life Science Osaka University

• Takano Kazufumi

  Department Of Material And Life Science Graduate School Of Engineering Osaka University

• Yoshimura Masashi

  Department Of Applied Biological Chemistry The University Of Tokyo

• Matsumura Hiroyoshi

  Department Of Applied Chemistry Graduate School Of Engineering Osaka University

• Morikawa Masaaki

  Department Of Biotechnology Department Of Material & Life Science Graduate School Of Engineering

• Mori Yusuke

  Department Of Applied Chemistry School Of Science And Engineering Waseda University

• Adachi Hiroaki

  Department Of Biological Science Faculty Of Science Hiroshima University

• Inoue Tsuyoshi

  Department Of Appied Chemistry Faculty Of Engineering Osaka University

• Sasaki Takatomo

  Department Of Electrical Electronic And Information Engineering Osaka University

• Masuhara Hiroshi

  Department of Applied Physics, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan

• Morikawa Masaaki

  Department of Material and Life Science, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan

• Kanaya Shigenori

  Department of Material and Life Science, Graduate School of Engineering, Osaka University

• Kanaya Shigenori

  Department of Material and Life Science, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan

• Inoue Tsuyoshi

  Department of Materials Chemistry, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan

• Tsunaka Yasuo

  Department of Material and Life Science, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan

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