Femtosecond Laser Processing of Protein Crystals in Crystallization Drop
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概要
- 論文の詳細を見る
We have developed a novel processing technique for protein crystals using femtosecond laser irradiation. We call this technique the femtosecond-laser-induced cut and cleave operation (fs-CACO). By precisely controlling the laser fluence and the position of the laser focal point, we are able to perform accurate and reproducible processing of protein crystals with little damage without troublesome treatment such as the unsealing of vessels and removal of solutions surrounding the crystals. To our knowledge, this is the first report on processing protein crystals using ultra-short-pulse laser ablation. Fs-CACO will be a powerful tool for making problematic protein crystals suitable for X-ray diffraction measurements.
- INSTITUTE OF PURE AND APPLIED PHYSICSの論文
- 2005-06-10
著者
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Takano Kazufumi
SOSHO Project (Crystal Design Project), Osaka University
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Matsumura Hiroyoshi
SOSHO Project (Crystal Design Project), Osaka University
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Inoue Tsuyoshi
SOSHO Project (Crystal Design Project), Osaka University
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MASUHARA Hiroshi
Department of Allied Physics,Osaka University
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KITANO Hiroshi
SOSHO Project (Crystal Design Project), Osaka University
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Mori Yusuke
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Adachi Hiroaki
Department Of Biological Science Faculty Of Science Hiroshima University
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Yoshikawa Hideki
Department Of Applied Physics Osaka University
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Sugamoto Kazuomi
Department Of Orthopaedic Biomaterial Science Osaka University Graduate School Of Medicine
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Sasaki Takatomo
Department Of Electrical Electronic And Information Engineering Osaka University
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Hosokawa Yoichiroh
Department of Applied Physics, Handai Frontier Research Center, and Venture Business Laboratory of Center for Advanced Science and Innovation, Osaka University, Suita, Osaka 565-0871, Japan
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Sasaki Takatomo
Department of Electrical Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
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Murakami Satoshi
SOSHO Inc., Osaka 541-0053, Japan
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Takano Kazufumi
SOSHO Project (Crystal Design Project), Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
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Matsumura Hiroyoshi
SOSHO Project (Crystal Design Project), Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
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Kashii Masafumi
Department of Electrical Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
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