Characterization of a Small Metalloprotease from Streptomyces caespitosus with High Specificity to Aromatic Residues

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概要

• 論文の詳細を見る

• A zinc metalloendoprotease from Streptomyces caespitosus (ScNP) is one of the smallest proteases found to data. It consists of a single polypeptide chain of 132 amino acid residues with a molecular weight of about 15 kDa. According to the amino acid sequence, this protease was classified in the Streptomyces small neutral protease family with an aspartate (Asp93) as a metal-binding site. We have determined the cleavage specificities and the optimal conditions for hydrolysis. The amino-terminal amino acid sequences of the hydrolyzed products of an oxidized insulin B-chain showed a high specificity to aromatic residues such as phenylalanine and tyrosine. The enzyme showed maximal activity against azocasein at pH 6.0 and 50℃. ScNP was inhibited by EDTA and 1,10-phenanthroline. Our studies indicated that ScNP is the smallest metalloendoprotease which hydrolyzes with high specificity and this specificity of ScNP was compared with those of other metalloendoproteases.

• 社団法人日本生物工学会の論文
• 1997-06-25

著者

• KAI Yasushi

  Department of Materials Chemistry, Graduate School of Engineering, Osaka University

• Kai Y

  Osaka Univ. Osaka

• 栗栖 源嗣

  大阪大学蛋白質研究所

• Takagi Masahiro

  School Of Materials Science Japan Advanced Institute Of Science And Technology

• Takagi M

  School Of Materials Science Japan Advanced Institute Of Science And Technology

• IMANAKA Tadayuki

  Department of Synthetic and Biological Chemistry, Graduate School of Engineering, Kyoto University

• TAKAGI MASAHIRO

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Kai Yasushi

  Department Of Applied Chemistry Faculty Of Engineering Osaka University

• Takagi M

  Nagasaki Univ. Nagasaki Jpn

• Imanaka Tadayuki

  Department Of Applied Biotechnology Faculty Of Engineering Osaka University

• HARADA Shigeharu

  Department of Applied Biology, Kyoto Institute of Technology

• Sugimoto Akiko

  Department Of Applied Chemistry Faculty Of Engineering Osaka University

• KURISU GENJI

  Department of Applied Chemistry, Faculty of Engineering, Osaka University

• Harada Shigeharu

  Department Of Applied Biology Kyoto Institute Of Technology

• Takagi Masahiro

  Department Of Biotechnology Faculty Of Engineering Osaka University

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