Expression of Recombinant Antibody against Cancer-Specific Carbohydrate

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概要

• 論文の詳細を見る

• Starting with a previously established hybridoma producing a monoclonal antibody (F1α75) against a synthetic glycolipid, in which the carbohydrate moiety was artificially designed to be putatively cancer-specific, we cloned complementary DNA (cDNA) for active variable regions of both heavy and light chains of the antibody. We established a Chinese hamster ovary (CHO) cell line expressing a recombinant F1α75 of human IgG class.The recombinant F1α75 retained the binding ability to its antigen and was easily purified. The result suggests that the conversion into the IgG class by genetic engineering is useful for antibodies of the IgM class which are difficult to be purified due to aggregation. Sequence analysis of variable regions of F1α75 revealed no strong homology with an antibody against galactose, the terminal residue of the synthetic glycolipid, suggesting the clinical usefulness of the recombinant F1α75.

• 社団法人日本生物工学会の論文
• 1995-05-25

著者

• Takagi Masahiro

  School Of Materials Science Japan Advanced Institute Of Science And Technology

• KANNAGI Reiji

  Laboratory of Experimental Pathology, Aichi Cancer Center Research Institute

• Kannagi R

  Aichi Cancer Center Res. Inst. Nagoya Jpn

• Takagi M

  School Of Materials Science Japan Advanced Institute Of Science And Technology

• Kannagi Reiji

  Department Of Molecular Pathology Research Institute Aichi Cancer Center

• Kannagi Reiji

  Laboratory Of Experimental Pathology Aichi Cancer Center Research Institute

• IMANAKA Tadayuki

  Department of Synthetic and Biological Chemistry, Graduate School of Engineering, Kyoto University

• TAKAGI MASAHIRO

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Takagi M

  Nagasaki Univ. Nagasaki Jpn

• Yasukawa K

  Tosoh Corp. Kanagawa Jpn

• Yasukawa Kiyoshi

  Scientific Instruments Division Tosoh Corporation

• Imanaka Tadayuki

  Department Of Applied Biotechnology Faculty Of Engineering Osaka University

• Kohda Katsunori

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Iba Yoshitaka

  Biotechnology Research Laboratory Tosoh Corporation

• TAKAGI Kuniaki

  Department of Environmental Biochemistry and Toxicology, University of Shizuoka School of Pharmaceut

• YASUKAWA KIYOSHI

  Biotechnology Research Laboratory, Tosoh Corporation

• ISOBE YUJI

  Department of Envirionmental Biochemistry, University of Shizuoka School of Pharmaceutical Science

• SUKETA YASUNOBU

  Department of Environmental Biochemistry, University of Shizuoka School of Pharmaceutical Science

• Isobe Yuji

  Department Of Envirionmental Biochemistry University Of Shizuoka School Of Pharmaceutical Science

• Takagi Kuniaki

  Department Of Environmental Biochemistry And Toxicology School Of Pharmaceutical Science University

• Takagi Kuniaki

  Department Of Environmental Biochemistry University Of Shizuoka School Of Pharmaceutical Science

• Suketa Yasunobu

  Department Of Environmental Biochemistry And Toxicology School Of Pharmaceutical Science University

• Suketa Yasunobu

  Department Of Environmental Biochemistry University Of Shizuoka School Of Pharmaceutical Science

• Takagi Masahiro

  Department Of Biotechnology Faculty Of Engineering Osaka University

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