Display of Heterologous Gene Products on the Escherichia coli Cell Surface as Fusion Proteins with Flagellin

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概要

• 論文の詳細を見る

• We have developed a system for expressing and displaying recombinant proteins, on the surface of Escherichia coli cells using the flagelling gene (hag). In order to produce fusion proteins, DNA fragments encoding a small peptide with 15 amino acid residues (P15), the constant region of the antibody L chain (region C), a single chain Fv (sFv) of an anti-porphyrin antibody, green fluorescent protein (GFP) and a bacterial alkaline phosphatase (BAP), respectively were inserted into the dispensable D3 domain encoded by hag. Each fusion gene was expressed in E.coli YK4516, a strain that lacks hag. The peptide linker P15 and the region C were efficiently expressed and displayed in the flagellar fraction as fusion proteins. Although with lower efficiency, sFv, BAP and GFP could also be expressed in the flagellar fraction. Therefore, this system is useful for epitope analysis and with some improvements, the method could be useful for the expression of heterologous proteins on the cell surface.

• 社団法人日本生物工学会の論文
• 1998-11-25

著者

• Takagi Masahiro

  School Of Materials Science Japan Advanced Institute Of Science And Technology

• Takagi M

  School Of Materials Science Japan Advanced Institute Of Science And Technology

• Takagi Mutsumi

  International Center For Biotechnology Osaka University:(present Address)division Of Biotechnology A

• IMANAKA Tadayuki

  Department of Synthetic and Biological Chemistry, Graduate School of Engineering, Kyoto University

• TAKAGI MASAHIRO

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• EZAKI SATOSHI

  Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto Un

• Takagi M

  Nagasaki Univ. Nagasaki Jpn

• Ezaki S

  Department Of Synthetic Chemistry And Biological Chemistry Graduate School Of Engineering Kyoto Univ

• Ezaki Satoshi

  Department Of Synthetic Chemistry And Biological Chemistry Graduate School Of Engineering Kyoto Univ

• Ezaki Satoshi

  Laboratory Of Molecular Gene Technics Graduate School Of Genetic Resources Technology Kyushu Univers

• Imanaka Tadayuki

  Department Of Applied Biotechnology Faculty Of Engineering Osaka University

• TSUKIO MANAE

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Imanaka T

  Dep. Of Biotechnology Coll. Of Life Sciences Ritsumeikan Univ.

• Tsukio Manae

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Takagi Masahiro

  Department Of Biotechnology Faculty Of Engineering Osaka University

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