Effect of Polyamines on Histone-Induced DNA Compaction of Hyperthermophilic Archaea
スポンサーリンク
概要
- 論文の詳細を見る
The effect of polyamines on histone-mediated DNA compaction was examined in vitro with archaeal histone HpkA from Pyrococcus kodakaraensis KOD1.An agarose gel mobility-shift experiment indicated that histone-bound DNA (compacted DNA) was further compacted by addition of a polyamine (putrescine, spermidine, or spermine) or its acetylated form (N-acetylputrescine, N^1-acetylspermidine, N^8-acetylspermidine, or N^1-acetylspermine) when the mixture was incubated at above 75℃. Spermine was most effective in compaction enhancement among all the polyamines tested. A high concentration of potassium ion (1.0M) did not stabilize the compacted form of DNA even though double-stranded DNA was stably maintained against thermal denaturation at elevated temperatures under this condition. It appears likely that multivalent polyamines have a nucleosome maintenance function in hyperthermophilic archaea in high-temperature environments.
- 社団法人日本生物工学会の論文
- 2000-01-25
著者
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Takagi Masahiro
School Of Materials Science Japan Advanced Institute Of Science And Technology
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FUKUI Kiichi
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Fukui Kiichi
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Takagi M
School Of Materials Science Japan Advanced Institute Of Science And Technology
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Takagi Mutsumi
International Center For Biotechnology Osaka University:(present Address)division Of Biotechnology A
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FUJIWARA SHINSUKE
Department of Bioscience, School of Science and Technology, Kwansei Gakuin University
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IMANAKA Tadayuki
Department of Synthetic and Biological Chemistry, Graduate School of Engineering, Kyoto University
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TAKAGI MASAHIRO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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HIGASHIBATA HIROKI
Department of Biotechnology, Graduate School of Engineering, Osaka University
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EZAKI SATOSHI
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto Un
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Fujiwara S
School Of Science And Engineering Department Of Life Science Kwansei-gakuin University
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Fujiwara Shinsuke
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Fujiwara Shinsuke
Department Of Bioscience School Of Science And Technology Kwansei Gakuin University
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Takagi M
Nagasaki Univ. Nagasaki Jpn
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Ezaki S
Department Of Synthetic Chemistry And Biological Chemistry Graduate School Of Engineering Kyoto Univ
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Ezaki Satoshi
Department Of Synthetic Chemistry And Biological Chemistry Graduate School Of Engineering Kyoto Univ
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Ezaki Satoshi
Laboratory Of Molecular Gene Technics Graduate School Of Genetic Resources Technology Kyushu Univers
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Imanaka Tadayuki
Department Of Applied Biotechnology Faculty Of Engineering Osaka University
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Hashimoto H
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Fukui Kiichi
Department Of Biotechnology Faculty Of Engineering Graduate School Of Osaka University
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Fujiwara Shinsuke
School Of Science And Engineering Department Of Life Science Kwansei-gakuin University
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Fujimoto K
Japan Advanced Inst. Of Sci. And Technol. Ishikawa
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Imanaka T
Dep. Of Biotechnology Coll. Of Life Sciences Ritsumeikan Univ.
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Takagi Masahiro
Graduate School Of Biological Sciences Nara Institute Of Science And Technology (naist)
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Takagi Masahiro
Department Of Biotechnology Faculty Of Engineering Osaka University
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Fujiwara Shinsuke
Department Of Bioscience Graduate School Of Science And Technology Kwansei-gakuin University:researc
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Higashibata Hiroki
Department Of Bioscience Graduate School Of Science And Technology Kwansei-gakuin University
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