Fluorescent labeling of plant chromosomes in suspension by FISH
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概要
- 論文の詳細を見る
By optimizing the concentration and time of treatment with hydroxyurea (HU), a DNA synthesis inhibitor, and trifluralin, a microtubule inhibitor, a highly effective (over 60%) cell cycle synchronization method for rye and barley meristem cells was developed. Chromosome suspensions containing highly purified and morphologically intact rye and barley chromosomes were prepared from the meristems of their root tips by homogenization. Digoxigenin-labeled 5S rDNA was used as a probe in FISH for the rye chromosomes in the suspension, and biotin-labeled 17S rDNA and centromeric DNA were used in FISH for the rye and barley chromosome suspensions, respectively. Bright signals were detected at the specific regions of interest on the chromosomes. The results indicate that the method developed in this study is useful for selection and sorting of chromosomes that are not distinguishable by other means, using specific fluorescent labeling by FISH of the chromosomes in suspension.
- 日本遺伝学会の論文
- 2005-02-01
著者
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FUKUI Kiichi
Department of Biotechnology, Graduate School of Engineering, Osaka University
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UCHIYAMA Susumu
Department of Electrical Engineering, Faculty of Engineering, Nagoya University
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Uchiyama S
Laboratory Of Dynamic Cell Biology Department Of Biotechnology Graduate School Of Engineering Osaka
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OHMIDO Nobuko
Faculty of Human Development, Kobe University
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MA Youzhi
Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, Institute of Crop Breeding an
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LEE Jai-Heon
Faculty of Natural Resources and Life Science, Dong-A University
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LI Lian
Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, Institute of Crop Breeding an
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Ohmido N
Graduate School Of Human Development And Environment Kobe University
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Ohmido Nobuko
Fac. Hum. Dev. Kobe Univ.
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Ohmido Nobuko
Faculty Of Human Development Kobe Univ.
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Fukui K
Grad. Sch. Of Eng. Osaka Univ.
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Lee Jai-heon
Faculty Of Natural Resources And Life Science Dong-a University
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Ma Youzhi
Key Laboratory Of Crop Genetics And Breeding Ministry Of Agriculture Institute Of Crop Breeding And
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Fukui Kiichi
Department Of Biotechnology Faculty Of Engineering Graduate School Of Osaka University
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Ohmido N
Faculty Of Human Development Kobe University
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Uchiyama S
Osaka Univ. Suita Jpn
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Uchiyama Susumu
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Li Lian
Key Laboratory Of Crop Genetics And Breeding Ministry Of Agriculture Institute Of Crop Breeding And
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Uchiyama Susumu
大阪大学 工学研究科生物工学コース
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