Image analysis of small plant chromosomes by using an improved system, CHIAS IV
スポンサーリンク
概要
- 論文の詳細を見る
Small plant chromosomes show uneven condensation during the prometaphase stage in mitosis. Recently, an improved chromosome image analyzing system (CHIAS IV) has been released for measurement of the condensation patterns (CPs) in fluorescence images. CHIAS IV is based on the multiplatform public-domain image-processing software, ImageJ, which was developed by the National Institutes of Health, US. In this study, we used CHIAS IV to perform image analysis of the fluorescence patterns of somatic prometaphase chromosomes of the leguminous plant, red clover (Trifolium pratense L., 2n = 2x = 14). We produced a quantitative chromosome map of red clover by combining the qualitative-analysis results obtained using fluorescence in situ hybridization (FISH) mapping with the results of the CHIAS IV image-analysis method, thereby clearly demonstrating the effectiveness and utility of this software as an analytical tool for genetic and genomic studies.
- 財団法人 染色体学会の論文
- 2009-07-01
著者
-
FUKUI Kiichi
Department of Biotechnology, Graduate School of Engineering, Osaka University
-
Fukui Kiichi
Department Of Biotechnology Graduate School Of Engineering Osaka University
-
KATO Seiji
Yamanashi Pref. Agri. Tech. Cen.
-
Hara Masaki
Graduate School Of Human Development And Environment Kobe University
-
Kato Seiji
Yamanashi Prefectural Agritechnology Center
-
Ohmido N
Graduate School Of Human Development And Environment Kobe University
-
Ohmido Nobuko
Fac. Hum. Dev. Kobe Univ.
-
Ohmido Nobuko
Graduate School Of Human Development And Environment Kobe University
-
Ohmido Nobuko
Graduate School Of Culture Study Human Science Kobe University
-
Kataoka Ryohei
Fac. Hum. Dev. Kobe Univ.
-
KATAOKA Ryouhei
Graduate School of Human Development and Environment, Kobe University
-
Fukui Kiichi
Department Of Biotechnology Faculty Of Engineering Graduate School Of Osaka University
-
Kataoka Ryouhei
Graduate School Of Human Development And Environment Kobe University
関連論文
- Comprehensive analysis of dynamics of histone H4 acetylation in mitotic barley cells
- Physical Mapping of Ribosomal DNA Genes on Jatropha curcas Chromosomes by Multicolor FISH
- Genome and Chromosome Dimensions of Lotus japonicus
- Anti-Peptide Antibodies for Examining the Conformation, Molecular Assembly and Localization of an Intracellular Protein, Ribosomal Protein S6, In vivo
- O-21. Human metaphase chromosomes viewed from the protein constituent(Abstracts for the oral and the poster presentations,Abstracts of the Joint Meeting: the 58th Annual Meeting of the Society of Chromosome Research and the 17th Annual Meeting of the Chro
- O-22. The effects of divalent cations on chromosome higher order structure and the localization of chromosomal proteins(Abstracts for the oral and the poster presentations,Abstracts of the Joint Meeting: the 58th Annual Meeting of the Society of Chromosom
- O-23. ASURA is involved in morphogenesis of X-shaped chromosomes(Abstracts for the oral and the poster presentations,Abstracts of the Joint Meeting: the 58th Annual Meeting of the Society of Chromosome Research and the 17th Annual Meeting of the Chromosom
- Functional analysis of plant Aurora kinase in mitosis
- The chromosomal protein SML plays a role in the regulation of stamen development
- Characterization of a Splicing Variant of Plant Aurora Kinase
- P-01. Aurora kinase is required for chromosome segregation in plants(Abstracts of the oral and poster presentations)
- O-26. A nucleolar protein, nucleolin, is involved in chromosome dynamics(Abstracts of the oral and poster presentations)
- O-25. Reconstitution of chromatin using human recombinant proteins(Abstracts of the oral and poster presentations)
- O-24. Localization analysis of the novel chromosomal protein identified using salt stripping method(Abstracts of the oral and poster presentations)
- O-23. Proteome analysis of human metaphase chromosomes(Abstracts of the oral and poster presentations)
- O-22. Chromosome scaffold and higher-order structure of metaphase chromosomes investigated by electron microscopy(Abstracts of the oral and poster presentations)
- Fluorescent labeling of plant chromosomes in suspension by FISH
- Application of the Bio-Active Beads Method in Rice Transformation
- Development of New Dosimetry Using Extended DNA Fibers(METHODS)
- Cell Culture in a Closed Nano-Space(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- An Arabidopsis thaliana Gene on the Yeast Artificial Chromosome Can Be Transcribed in Tobacco Cells
- Changes in Chromosomal Surface Structure by Different Isolation Conditions
- O-15. Construction of red clover (Trifolium pratense L.) chromosome map(Abstracts for the oral and the poster presentations,Abstracts of the Joint Meeting: the 58th Annual Meeting of the Society of Chromosome Research and the 17th Annual Meeting of the Ch
- P-04. Establishment of red clover chromosome map by the improved chromosome image analyzing system, CHIAS4(Abstracts for the oral and the poster presentations,Abstracts of the Joint Meeting: the 58th Annual Meeting of the Society of Chromosome Research an
- O-32. Integration of linkage and chromosome maps of red clover(Trifolium pratense L.)(Abstracts of the oral and poster presentations)
- Chromosome maps of legumes
- Direct Amplification of an α-Amylase Gene from Barley Chromosome 6 by a Microdissection Method
- Detection of Proteins Binding to the Promoter Region DNA Using a Nonradioactive Gel-Retardation Assay
- Cellular Toxicity of Cadmium Ions and Their Detoxification by Heavy Metal-Specific Plant Peptides, Phytochelatins, Expressed in Mammalian Cells^1
- Utilization of Immobilized Archaeal Chaperonin for Enzyme Stabilization
- Enhanced Signal Transduction by a Directly Fused Protein of Interleukin-6 and Its Receptor
- Unique Nucleoid Structure during Cell Division of Thermococcus kodakaraensis KOD1
- Alteration of Product Specificity of Cyclodextrin Glucanotransferase from Thermococcus sp.B1001 by Site-Directed Mutagenesis
- Effect of Polyamines on Histone-Induced DNA Compaction of Hyperthermophilic Archaea
- Characterization; Genome Sizes and Morphology of Sex Chromosomes in Hemp (Cannabis sativa L.)
- Quantitative Chromosome Map of Arabidopsis thaliana L.by Imaging Methods
- Chromosome research of legume plants(Joint Symposium of the Society of Chromosome Research and the Chromosome Colloquium : "Dynamics of genome and chromosomes-aspects from gene expression to evolution-",Abstracts of the 57th Annual Meeting of the Society
- Fertile somatic hybrids between Solanum integrifolium and S. sanitwongsei (syn. S. kurzii) as candidates for bacterial wilt-resistant rootstock of eggplant
- Estimation of the Nuclear DNA Content of Strawberries (Fragaria spp.) Compared with Arabidopsis thaliana by Using Dual-step Flow Cytometry
- Image analysis of small plant chromosomes by using an improved system, CHIAS IV
- O-16. The characteristic condensation patterns in rice chromosome(Abstracts for the oral and the poster presentations,Abstracts of the Joint Meeting: the 58th Annual Meeting of the Society of Chromosome Research and the 17th Annual Meeting of the Chromoso
- Development of a quantitative pachytene chromosome map in Oryza sativa by imaging methods
- Comparison of Surface Structures between Extended and Condensed Stages of Barley Chromosomes Revealed with Atomic Force Microscopy
- ESTIMATION OF COPY NUMBER BY HIGH RESOLUTION FISH USING EXTENDED DNA FIBERS IN RICE
- Advances in rice chromosomes research
- Improvement of Transformation Efficiency by Bioactive-Beads-Mediated Gene Transfer Using DNA-Lipofectin Complex as Entrapped Genetic Material(PLANT BIOTECHNOLOGY)
- A Novel Gene Delivery System in Plants with Calcium Alginate Micro-Beads(PLANT BIOTECHNOLOGY)
- Estimation of the Copy Number of Ribosomal RNA Genes by a Photon-counting Method
- Multi-Spectral Two-Photon Excited Fluorescence Microscopy Using Supercontinuum Light Source
- A Novel Transfection Method for Mammalian Cells Using Calcium Alginate Microbeads (TISSUE ENGINEERING)
- 1P035 3α-hydroxysteroid dehydrogenaseの二量体界面にあるArg251とPhe255の役割(蛋白質-構造機能相関,第48回日本生物物理学会年会)
- Obtaining transgenic plants using the bio-active beads method
- O-26. Entrapment of HeLa cell chromosome in bioactive beads(Abstracts for the oral and the poster presentations,Abstracts of the Joint Meeting: the 58th Annual Meeting of the Society of Chromosome Research and the 17th Annual Meeting of the Chromosome Col
- Protection of isolated chromosome using bioactive beads toward physical and chemical stresses
- Chromatin Arrangements in Intact Interphase Nuclei Examined by Laser Confocal Microscopy
- An upper limit of the ratio of DNA volume to nuclear volume exists in plants
- Physical Mapping of 18S rDNA by Fluorescence in situ Hybridization (FISH) in the Three Species of the Genus Paris L., Liliaceae
- Adventitious shoot regeneration from juvenile cotyledons of a biodiesel producing Plant, Jatropha curcas L(PLANT BIOTECHNOLOGY)
- Development of novel humanized anti-CD20 antibodies based on affinity constant and epitope
- Improvement of bioactive bead-mediated transformation by concomitant application of electroporation
- Polymorphism in Rice Amylases at an Early Stage of Seed Germination
- 間接蛍光抗体法を用いたダイズ染色体の複製パタ-ンの解析〔英文〕
- WS1-2 Characteristics and analysis methods for small chromosomes With special reference to genera, Oryza and Brassica(Workshop 1: "From large chromosomes to small chromosomes: Wide varieties of the genome and chromosome sizes",Abstracts of the Joint Meeti
- SY-8 Three-dimensional analysis of histone acetylation in barley(Symposium: "The past, present and future studies of the Genome, the Chromatin and the Chromosome",Abstracts of the Joint Meeting: the 58th Annual Meeting of the Society of Chromosome Researc
- Recent advances in rice genome and chromosome structure research by fluorescence in situ hybridization (FISH)
- Interstitial Telomere-like Repeats in the Haplopappus gracilis (Asteraceae) Genome Revealed by Fluorescence in situ Hybridization
- RICE CHROMOSOMES STUDIES BY FLUORESCENCE IN SITU HYBRIDIZATION WITH SPECIAL REFERENCE TO PHYSICAL MAPPING AND CHROMOSOME STRUCTURE
- Live Cell Imaging Reveals Plant Aurora Kinase Has Dual Roles During Mitosis
- Dual-Band Stimulated Parametric Emission Microscopy
- Three-Dimensional Unstained Live-Cell Imaging Using Stimulated Parametric Emission Microscopy
- Nonlinear Phase Imaging Using Two-Beam Interferometry in Stimulated Parametric Emission Microscopy
- A Yeast Artificial Chromosome-Splitting Vector Designed for Precise Manipulation of Specific Plant Chromosome Region(Genetics, Molecular Biology, and Gene Engineering)
- Repeated Chromosome Splitting Targeted to δ Sequences in Saccharomyces cerevisiae
- Fc domain mediated self-association of an IgG1 monoclonal antibody under a low ionic strength condition(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- 2La12 Phylogenetic analysis of Ty1-copia retrotransposons in Jatropha curcas L.
- 2D1536 3α-hydroxysteroid dehydrogenaseの二量体形成能と酵素機能との相関(蛋白質_構造機能相関1,第49回日本生物物理学会年会)
- Subharmonic Synchronization of Picosecond Yb Fiber Laser to Picosecond Ti:Sapphire Laser for Stimulated Raman Scattering Microscopy
- An X-chromosome RNA-binding motif protein (RBMX) is required for proper kinetochore formation
- Highly Sensitive Signal Detection in Stimulated Parametric Emission Microscopy Based on Two-Beam Interferometry
- Direct Amplification of an α-Amylase Gene from Barley Chromosome 6 by a Microdissection Method
- Condensation pattern as a new image parameter for identification of small chromosomes in plants.
- Localization of transgene-derived friabilins in rice endosperm cells
- Multi-Spectral Two-Photon Excited Fluorescence Microscopy Using Supercontinuum Light Source
- Detection of sister chromatid exchange sites by sequential staining and imaging methods.
- Cellular Toxicity of Cadmium Ions and Their Detoxification by Heavy Metal-Specific Plant Peptides, Phytochelatins, Expressed in Mammalian Cells.