A Novel Gene Delivery System in Plants with Calcium Alginate Micro-Beads(PLANT BIOTECHNOLOGY)

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概要

• 論文の詳細を見る

• We have produced micrometer-sized calcium alginate beads referred to as "bio-beads" that encapsulate plasmid DNA molecules carrying a reporter gene. In order to evaluate the efficiency of the bio-beads in mediating genetic transfection, protoplasts isolated from cultured tobacco cells (BY-2) were transfected with bio-beads containing a plasmid that carries the modified green fluorescent protein gene CaMV35S-sGFP. With the bio-beads treatment, approximately ten-fold higher GFP expression was observed after 24 h incubation compared to that with the conventional method using a naked plasmid solution. Transfection was up to 0.22% efficient. These results indicate that bio-beads have a possibility for efficient transformation in plants.

• 社団法人日本生物工学会の論文
• 2002-07-25

著者

• Harashima Satoshi

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Nagamori Eiji

  Department Of Biotechnology School Of Engineering Nagoya University

• Nagamori Eiji

  Toyota Central R & D Labs. Inc.

• Nagamori E

  Osaka Univ. Osaka Jpn

• Nagamori Eiji

  Biotechnology Laboratory Toyota Central R&d Labs Inc.

• FUKUI Kiichi

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• FUKUSAKI EI-ICHIRO

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• KOBAYASHI AKIO

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Nagamori Eiji

  Toyota Central R&d Labs. Inc. 41-1 Yokomichi Nagakute Aichi 480-1192 Jpn

• KAWABE Akira

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• SONE Takefumi

  Department of Biotechnology, Osaka University, Graduate School of Engineering

• Kobayashi A

  National Inst. Advanced Industrial Sci. And Technol. Ibaraki Jpn

• Ikeuchi T

  Research Institute For Marine Cargo Transportation Kobe University Of Mercantile Marine

• Ikeuchi Tomohiko

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Kobayashi A

  Laboratory Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• MIZUKAMI ATSUSHI

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• TAKAKURA YUKIKO

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Fukui K

  Grad. Sch. Of Eng. Osaka Univ.

• Harashima S

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Harashima Satoshi

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Sone Takefumi

  Imbd Osaka Univ.

• Fukui Kiichi

  Department Of Biotechnology Faculty Of Engineering Graduate School Of Osaka University

• Fukusaki Ei-ichiro

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Fukusaki Eiichiro

  Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.

• Fukusaki Ei-ichiro

  大阪大学大学院

• Kajiyama Shin'ichiro

  Department Of Biotechnology Graduate School Of Engineering Osaka University:school Of Biology-orient

• Kajiyama Shin'ichiro

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Fukusaki E

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Kajiyama Shin-ichiro

  Faculty Of Agriculture Okayama University

• Mizukami Atsushi

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Takakura Yukiko

  大阪大学大学院

• Harashima Satoshi

  Dept. Biotech. Grad. Sch. Eng. Osaka Univ.

• Kobayashi Akio

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Nagamori Eiji

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• KOBAYASHI Akio

  Department of Biochemistry, Shinshu University School of Medicine

• KOBAYASHI Akio

  Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University

• HARASHIMA Satoshi

  Department of Biotechnoiogy, Graduate School of Engineering, Osaka University

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