Construction and Characterization of Single-Gene Chromosomes in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)

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概要

• 論文の詳細を見る

• Based on a previously developed PCR-mediated chromosome splitting method, a genome engineering technique was developed in haploid Saccharomyces cerevisiae for incorporating any desired chromosomal region into a chromosome that carries a single gene. Based on the viability of cells carrying an essential gene in such a construct, close physical proximity of two telomeres and a centromere does not appear to compromise gene function. Spontaneous loss of constructed singlegene chromosomes during vegetative growth was high (0.2-0.4per cell division), suggesting the possibility of creating novel cells carrying single-gene chromosomes derived from various chromosomal regions in a variety of combinations by exploiting combinatorial loss.

• 社団法人日本生物工学会の論文
• 2008-12-25

著者

• Yamagishi Kazuo

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Sugiyama Minetaka

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Kaneko Yoshinobu

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Harashima Satoshi

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Harashima S

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Harashima Satoshi

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Kaneko Y

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Kaneko Yoshinobu

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Nishizawa Masafumi

  Department of Microbiology and Immunology, Keio University School of Medicine

• Yamagishi Kazuo

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Kaneko Yoshinobu

  Dept. Biotech. Grad. Sch. Eng. Osaka Univ.

• Harashima Satoshi

  Dept. Biotech. Grad. Sch. Eng. Osaka Univ.

• Sugiyama M

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Sugiyama Minetaka

  Department Of Biochemical Engineering & Science Kyushu Institute Of Technology

• Nishizawa M

  Department Of Microbiology And Immunology Keio University School Of Medicine

• Nishizawa Masafumi

  Department Of Microbiology And Immunology Keio University School Of Medicine

• Minetaka Sugiyama

  Dept. Biotech. Grad. Sch. Eng. Osaka Univ.

• KANEKO Yoshinobu

  Department of Biotechnoiogy, Graduate School of Engineering, Osaka University

• HARASHIMA Satoshi

  Department of Biotechnoiogy, Graduate School of Engineering, Osaka University

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