A Simple Method for Detection of Enzyme Activities Involved in the Initial Step of Phthalate Degradation in Microorganisms
スポンサーリンク
概要
- 論文の詳細を見る
We have developed a simple method for the detection of phthalate 4,5-dioxygenase and 4,5-dihydro-4,5-dihydroxyphthalate dehydrogenase activities in the initial step of phthalate degradation in bacteria. It was found that cells of a Pseudomonas putida strain adapted for phthalate could convert quinolinic acid to a hydroxylated product having λ_<max> at 3.15 nm. The occurrence of this compound was visualized by reaction with diazotized p-nitroaniline with which a red compound having λ_<max> at 512 nm was produced. In practice, if cells in colonies developed on an agar plate containing mineral salt medium supplemented with 0.4% of disodium phthalate and 0.1% of quinolinic acid are active with respect to the 4,5-dihydroxyphthalate pathway, then the colonies would be colored red immediately upon spraying with the diazotized p-nitroaniline reagent. The method was used to identify the phthalate degradative pathway for 27 phthalate-utilizing a strains of the genera Pseudomonas (18 strains), Agrabacterium (3 strains), Alcaligenes (5 strains), and Micrococcus (1 strain). It was found that 24 of the 26 Gram-negative bacteria have the 4,5-dihydroxyphthalate pathway and that the remaining two strains of Pseudomonas sp. may metabolize via an unidentified pathway. other than the dihydroxy-phthalate pathways, and the Gram-positive strain of Micrococcus sp. metabolizes phthalate via the 3,4-dihydroxyphthalate pathway.
- 社団法人日本生物工学会の論文
- 1989-04-25
著者
-
Harashima Satoshi
Department of Biotechnology, Graduate School of Engineering, Osaka University
-
Harashima Satoshi
Department Of Biotechnology Faculty Of Engineering Osaka University
-
OSHIMA YASUJI
Department of Biotechnology, Faculty of Engineering, Osaka University
-
Nomura Yasuo
The Faculty Of Engineering Kansai University
-
Oshima Y
Faculty Of Engineering Kansai University
-
Oshima Yasuji
Department Of Biotechnology Faculty Of Engineering Osaka University
-
NOMURA YASUTOSHI
(Present address)Tokyo Research Laboratory, Japan Synthetic Rubber Co., Ltd.,
-
Nomura Yasutoshi
(present Address)tokyo Research Laboratory Japan Synthetic Rubber Co. Ltd.
-
Oshima Yasuji
Department Of Biotechnology Faculty Of Engineering Kansai University
関連論文
- Deciphering cellular functions of protein phosphatases by comparison of gene expression profiles in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Application of the Bio-Active Beads Method in Rice Transformation
- An Arabidopsis thaliana Gene on the Yeast Artificial Chromosome Can Be Transcribed in Tobacco Cells
- 3P-1006 Characterization of a suppressor of Δrrn10 disruptant causing the defect in rRNA transcription in Saccharomyces cerevisiae
- 2Bp14 Molecular breeding of Saccharomyces cerevisiae strain with high amount of RNA
- A Novel Gene Delivery System in Plans with Calcium Alginate Micro-Beads
- A Novel Gene Delivery System in Plants with Calcium Alginate Micro-Beads(PLANT BIOTECHNOLOGY)
- Obtaining transgenic plants using the bio-active beads method
- Fatty Acid Desaturation in Methylotrophic Yeast Hansenula polymorpha Strain CBS 1976 and Unsaturated Fatty Acid Auxotrophic Mutants
- Mass Mating Method in Combination with G418- and Aureobasidin A-Resistance Markers for Efficient Selection of Hybrids from Homothallic Strains in Saccharomyces cerevisias
- Genes in PHT Plasmid Encoding the Initial Degradation Pathway of Phthalate in Pseudomonas putida
- 3P-1005 Physiological characterization of high-temperature tolerant Saccharomyces cerevisiae strain and identification of a responsible gene
- Construction and Characterization of Single-Gene Chromosomes in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Creating a Saccharomyces cerevisiae Haploid Strain Having 21 Chromosomes(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Use of the PDR4 Gene as a Dominant Selective Marker in Combination with Cerulenin for Prototrophic Strains in Sacchromyces cerevisiae
- Simple Monitoring System for R-Mediated Site-specific Recombination on Chromosomes in Saccharomyces cerevisiae
- Construction of Host-Vector System in the Osmophilic Haploid Yeast Zygosaccharomyces rouxii
- A Method for Direct Selection of Mating-Competent Clones from Mating-Incompetent Industrial Strains of Saccharomyces cerevisiae
- MASCOTS II: A Dialog Manager in General Interface for Speech Input and Output (Special Issue on Speech and Discourse Processing in Dialogue Systems)
- A System for Temperature-Controlled Expression of a Foreign Gene with Dual Mode in Saccharomyces cerevisiae
- Improvement and Application of a Promoter-Probe Vector Bearing the PHO5 Gene as the Indicator Marker in Saccharomyces cerevisiae
- Partial Restoration of Sporulation Defect in Sake Yeasts, Kyokai No. 7 and No. 9,by Increased Dosage of the IME1 Gene
- The Transcriptional Activators of the PHO Regulon, Pho4p and Pho2p, Interact Directly with Each Other and with Components of the Basal Transcription Machinery in Saccharomyces cerevisiae^1
- 1019 Cloning and characterization of Δ 9-fatty acid desaturase genes from Pichia, Yarrowia and Kluyveromyces
- Tree-Based Approaches to Automatic Generation of Speech Synthesis Rules for Prosodic Parameters : Special lssue on Speech Synthesis: Current Technologies and Equipment
- PHT, a Transmissible Plasmid Responsible for Phthalate Utilization in Pseudomonas putida
- A Simple Method for Detection of Enzyme Activities Involved in the Initial Step of Phthalate Degradation in Microorganisms
- Interactive Synthesis of Conceptual Schema Based on Queries : Towards an Expert System of Relational Database Design
- Genetic Characterization of rbt Mutants That Enhance Basal Transcription from Core Promoters in Saccharomyces cerevisiae^1
- Control of Secretory Production of Human Lysozyme from Saccharomyces cerevisiar by Incubation Temperature and Phosphate Concentration
- Identification and Functional Characterization of Yeast ζ-COP
- Saccharomyces cerevisiae protein phosphatase Ppz1 and protein kinases Sat4 and Ha15 are involved in the control of subcellular localization of Gln3 by likely regulating its phosphorylation state(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Application of the PHO5-Gene-Fusion Technology to Molecular Genetics and Biotechnology in Yeast
- A Method for Fusing Chromosomes in Saccharomyces cerevisiae
- One-Step Splitting of a Chromosome in Haploid Cells of Saccharomyces cerevisiae and Its Effect on the Cell Proliferation
- One-step splitting of a chromosome in haploid cells of Saccharomyces cerevisiae
- Strategy for preventing bacterial contamination by adding exogenous ethanol in solid-state semi-continuous bioethanol production(BIOCHEMICAL ENGINEERING)
- Construction of a Saccharomyces cerevisiae strain with a high level of RNA(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Tetraploid Formation through the Conversion of the Mating-type Alleles by the Action of Homothallic Genes in the Diploid Cells of Saccharomyces Yeasts
- Isolation and Identification of Phthalate-Utilizing Bacteria
- 317 Factors Encoded by and Affecting the Holding Stability of Yeast Plasmid, pSR1.
- 314 Functional organization of yeast plasmid pSR1
- 408 Molecular organization of yeast plasmid, pSRI
- A Yeast Artificial Chromosome-Splitting Vector Designed for Precise Manipulation of Specific Plant Chromosome Region(Genetics, Molecular Biology, and Gene Engineering)
- Repeated Chromosome Splitting Targeted to δ Sequences in Saccharomyces cerevisiae
- The phosphatase system in Saccharomyces cerevisiae
- Lactic-acid stress causes vacuolar fragmentation and impairs intracellular amino-acid homeostasis in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Large-scale genome reorganization in Saccharomyces cerevisiae through combinatorial loss of mini-chromosomes(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Increased transcription of NOP15, involved in ribosome biogenesis in Saccharomyces cerevisiae, enhances the production yield of RNA as a source of nucleotide seasoning(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Characterization and gene expression profiles of thermotolerant Saccharomyces cerevisiae isolates from Thai fruits(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Lactic-acid stress causes vacuolar fragmentation and impairs intracellular amino-acid homeostasis in Saccharomyces cerevisiae
- Genetic interactions of ribosome maturation factors Yvh1 and Mrt4 influence mRNA decay, glycogen accumulation, and the expression of early meiotic genes in Saccharomyces cerevisiae
- Construction and Characterization of Single-Gene Chromosomes in Saccharomyces cerevisiae
- Large-scale genome reorganization in Saccharomyces cerevisiae through combinatorial loss of mini-chromosomes
- Enhanced bio-ethanol production from cellulosic materials by semi-simultaneous saccharification and fermentation using high temperature resistant Saccharomyces cerevisiae TJ14(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Increased transcription of NOP15, involved in ribosome biogenesis in Saccharomyces cerevisiae, enhances the production yield of RNA as a source of nucleotide seasoning
- Functionally redundant protein phosphatase genes PTP2 and MSG5 co-regulate the calcium signaling pathway in Saccharomyces cerevisiae upon exposure to high extracellular calcium concentration(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Disruption of multiple genes whose deletion causes lactic-acid resistance improves lactic-acid resistance and productivity in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Characterization and gene expression profiles of thermotolerant Saccharomyces cerevisiae isolates from Thai fruits
- Increased transcription of RPL40A and RPL40B is important for the improvement of RNA production in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Suppression mechanism of the calcium sensitivity in Saccharomyces cerevisiae ptp2Δmsg5Δ double disruptant involves a novel HOG-independent function of Ssk2, transcription factor Msn2 and the protein kinase A component Bcy1(GENETICS, MOLECULAR BIOLOGY, AND
- Functionally redundant protein phosphatase genes PTP2 and MSG5 co-regulate the calcium signaling pathway in Saccharomyces cerevisiae upon exposure to high extracellular calcium concentration