Mass Mating Method in Combination with G418- and Aureobasidin A-Resistance Markers for Efficient Selection of Hybrids from Homothallic Strains in Saccharomyces cerevisias

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概要

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• We have developed a mass mating method using the spore suspensions of homothallic yeasts of Saccharomyces cerevisiae in combination with dominant selective drug resistance markers, Tn601(903) against geneticin and AUR1-C against aureobasidin A for the selection of the hybrids. To examine the effectiveness of these markers in the mass mating method, each marker was introduced into a homothallic wine yeast. Using a mixed culture of spore suspensions from the resultant transformants, many hybrids were screened by the drug resistance markers. This method is more practical than the spore-to-spore mating method because it does not require the use of a micromanipulator and many hybrids are obtained at one time. The resultant hybrids could be utilized for industrial brewing because plasmids, which are used to confer resistance markers, are easily eliminated from the hybrids by cultivation in a medium without drugs. We propose that the mass mating method using spore suspensions in combination with dominant selective geneticin- and aureobasidin A-esistance markers is useful for the selection of hybrieds from industrial homothallic yeasts.

• 社団法人日本生物工学会の論文
• 1999-11-25

著者

• Harashima Satoshi

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Nakazawa Nobushige

  Department of Biotechnology, Faculty of Bioresource Science, Akita Prefectural University

• Sato Takahide

  Department Of Bioproduction Science Faculty Of Horticulture Chiba University

• Enei H

  Iwate Biotechnol. Res. Center Iwate Jpn

• Enei Hitoshi

  Iwate Biotechnology Research Center

• Sato Takako

  The Deepstar Group Japan Marine Science And Technology Center

• Sato T

  Institute Of Applied Biochemistry University Of Tsukuba

• Enei H

  Iwate Biotechnology Research Center

• Harashima Satoshi

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Nakazawa Nobushige

  Department Of Biotechnology Faculty Of Bioresource Science Akita Prefectural University

• SATO Toshitsugu

  Iwate Biotechnology Research Center

• OKAWA Kumiko

  Iwate Biotechnology Research Center

• Sato T

  Iwate Biotechnology Research Center:department Of Biotechnology And Environmental Chemistry National

• Sato Tsutomu

  Graduate School Of Science And Technology Faculty Of Agriculture Niigata University

• Nakazawa Nobushige

  Department Of Applied Biology Faculty Of Bioresource Sciences Akita Prefectural University

• Okazawa Kumiko

  Iwate Biotechnology Research Center

• HARASHIMA Satoshi

  Department of Biotechnoiogy, Graduate School of Engineering, Osaka University

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