Purification and Some Properties of Alanine Aminotransferase from Candida maltosa
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概要
- 論文の詳細を見る
Alanine aminotransferase (AlaAT ; EC 2. 6. 1. 2) was purified to homogeneity from Candida maltosa that was grown on L-alanine as the sole source of carbon and nitrogen. The enzyme has a molecular mass of 99 kDa and consists of two subunits of equal molecular mass (52 kDa). Each subunit binds one mole of PLP. The enzyme has an isoelectric point of 5. 3 and an optimum pH of 6. 0-7. 5. The spectroscopic profile and an inhibition experiment showed that both PLP and free-SH groups are directly involved in the enzymatic catalysis. In the N-terminal region of the enzyme, the consensus sequence (five amino acids) that commonly appears in mammalian and higher plant enzymes is present. Compared with mammalian enzyme, the Candida AlaAT is heat-sensitive and a little looser in substrate specificity, and its affinity towards L-alanine is high.
- 社団法人日本農芸化学会の論文
- 1994-02-23
著者
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Sato Takahide
Department Of Bioproduction Science Faculty Of Horticulture Chiba University
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Sato Takako
The Deepstar Group Japan Marine Science And Technology Center
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KOMATSUBARA SABURO
Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co.Ltd.
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Sato T
Institute Of Applied Biochemistry University Of Tsukuba
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Komatsubara Saburo
Discovery Research Laboratory
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Komatsubara Saburo
Research Laboratory Of Applied Biochemistry Tanabe Seiyaku Co. Ltd.
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Umemura Isao
Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd.
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Yanagiya Koji
Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd.
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Sato Tadashi
Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd.
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Tosa Tetsuya
Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd.
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Tosa T
Research Laboratory Of Applied Biochemistry Tanabe Seiyaku Co. Ltd.
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Tosa Tetsuya
Research Laboratory Of Applied Biochemistry Tanabe Seiyaku Co. Ltd.
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Umemura Isao
Research Laboratory Of Applied Biochemistry Tanabe Seiyaku Co. Ltd.
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Umemura Isao
Research Institute For Fundamental Physics Kyoto University
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Yanagiya Koji
Research Laboratory Of Applied Biochemistry Tanabe Seiyaku Co. Ltd.
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Sato T
Iwate Biotechnology Research Center:department Of Biotechnology And Environmental Chemistry National
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Sato Tsutomu
Graduate School Of Science And Technology Faculty Of Agriculture Niigata University
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