Purification and Properties of Tyrosinase Isozymes from the Gill of Lentinus edodes Fruiting Body

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概要

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• Six tyrosinase isozymes were purified from the browned gill of the fruiting body of Lentinus edodes by ammonium sulfate fractionation, DEAE-Sephacel and Q-Sepharose column chromatography, and partially denaturing SDS-PAGE. At the stop of Q-Sopharose column chromatography, two active fractions (A and B) were obtained. Each fraction was separated to three further fractions, A1, A2, and A3, and B1, B2, and B3, respectively, by partially denaturing SDS-PAGE. All these isozymes consisted of two types of polypeptides: α polypeptide (Aα or Bα) and either β (Aβ or Bβ) or γ polypeptide (Aγ or Bγ). The αpolypeptide contained the consensus amino acid sequence of the active site of known tyrosinases, which is considered to act as a catalytic subunit. From the results of peptide mapping and the amino acid composition, Aα and Bα polypeptides were considered to be different proteins. The kinetic properties of the purified tyrosinase isozymes differed greatly according to whether they contained β or γ polypeptide, indicating these polypeptides to be a possible regulatory subunit.

• 社団法人日本農芸化学会の論文
• 1996-08-23

著者

• Sato Takahide

  Department Of Bioproduction Science Faculty Of Horticulture Chiba University

• Enei H

  Iwate Biotechnol. Res. Center Iwate Jpn

• Enei Hitoshi

  Iwate Biotechnology Research Center

• Sato Takako

  The Deepstar Group Japan Marine Science And Technology Center

• Sato T

  Institute Of Applied Biochemistry University Of Tsukuba

• Enei H

  Iwate Biotechnology Research Center

• Ejiri Shin-ichiro

  Institute for Cell Biology and Genetics, Faculty of Agriculture, Iwate University

• SATO Toshitsugu

  Iwate Biotechnology Research Center

• KANDA Katsuhiro

  Iwate Biotechnology Research Center

• ISHII Shizuko

  Iwate Biotechnology Research Center

• Ishii S

  San-ei Sucrochemical Co. Ltd. Chiba Jpn

• Ejiri Shin-ichiro

  Institute For Cell Biology And Genetics Iwate University

• Ejiri Shin-ichiro

  Institute For Cell Biology And Genetics Faculty Of Agriculture Iwate University

• Sato T

  Iwate Biotechnology Research Center:department Of Biotechnology And Environmental Chemistry National

• Sato Tsutomu

  Graduate School Of Science And Technology Faculty Of Agriculture Niigata University

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