Isolation of Genes Specifically Expressed in the Fruit Body of the Edible Basidiomycete Lentinula edodes

スポンサーリンク

概要

• 論文の詳細を見る

• In order to isolate the genes expressed specifically and abundantly in the mature fruit body of Lentinula edodes, the cDNAs derived from the gill of the fruit body were compared with the cDNAs from the mycelia by differential screening. Consequently, six clones were identified as fruit-body-specific genes (fbg03, 08, 13, 14, 16, and 21). The deduced amino acid sequence of fbg14 (Le.cypfb) had significant homology with the cytochrome P450 protein. The transcriptional level of fbg16, which showed 29.9% identity with the riboflavin aldehyde-forming enzyme of Agaricus bisporus, was highest among all of the fbg clones. This result indicates that the promoter region of fbg16 may become a powerful candidate for the expression signal of the vector for the gene manipulation in the mature fruit body.

• 社団法人 日本農芸化学会の論文
• 2004-02-23

著者

• Sato Takahide

  Department Of Bioproduction Science Faculty Of Horticulture Chiba University

• Hirano T

  Kyoto Univ. Kyoto Jpn

• Enei H

  Iwate Biotechnol. Res. Center Iwate Jpn

• Enei Hitoshi

  Iwate Biotechnology Research Center

• Sato Takako

  The Deepstar Group Japan Marine Science And Technology Center

• Sato T

  Institute Of Applied Biochemistry University Of Tsukuba

• Enei H

  Iwate Biotechnology Research Center

• Hirano Tatsuya

  Faculty of Agriculture, Meijo University

• SATO Toshitsugu

  Iwate Biotechnology Research Center

• Hirano Tatsuya

  Faculty Of Agriculture Meijo University

• Sato T

  Iwate Biotechnology Research Center:department Of Biotechnology And Environmental Chemistry National

• Taniyama Hiroko

  Department Of System Engineering Of Materials And Life Science Faculty Of Engineering Toyama Univers

• Horikawa Takashi

  Collaborative Research Center For Advanced Science And Technology Osaka University

• Sato Tsutomu

  Graduate School Of Science And Technology Faculty Of Agriculture Niigata University

関連論文

• Cyclic and Linear Peptides Derived from α-Amylase Inhibitory Protein Tendamistat^1
• Factors Improving L-Threonine Production by a Three L-Threonine Biosynthetic Genes-amplified Recombinant Strain of Brevibacterium lactofermentum
• High Fermentative Production of L-Threonine from Acetate by a Brevibacterium flavum Stabilized Strain Transformed with a Recombinant Plasmid Carrying the Escherichia coli thr Operon
• Application of a Recombinant Plasmid Expressing Desensitized 3-Deoxy-D-Arabinoheptulosonate-7-Phosphate Synthase to the Breeding of L-Phenylalanine Producers
• Improvement in Microbial Production of L-Tyrosine by Gene Dosage Effect of AroL Gene Encoding Shikimate Kinase(Microbiology & Fermentation Industry)
• Cloning and Characterization of Genes Responsible for m-Fluoro-D,L-phenylalanine Resistance in Brevibacterium lactofermentum(Microbiology & Fermentation Industry)
• Genetic Breeding of L-Tyrosine Producer from Brevibacterium lactofermentum(Microbiology & Fermentation Industry)
• Improvement of an L-Threonine Producer Derived from Brevibacterium flavum Using Threonine Operon of Escherichia coli K-12(Microbiology & Fermentation Industry)
• A New Cytotoxic Cholestane Bisdesmoside from Ornithogalum saundersiae Bulbs
• Aculeoside A, a Novel Steroidal Saponin Containing a Deoxyaldoketose from Ruscus aculeatus
• Inhibition of Proteasome by MG-132 Treatment Causes Extra Phragmoplast Formation and Cortical Microtubule Disorganization during M/G_1 Transition in Synchronized Tobacco Cells
• Cell-Cycle Dependent Dynamic Change of 26S Proteasome Distribution in Tobacco BY-2 Cells
• RECONSTRUCTION ( in vitro ) OF 20S PROTEASOME AND REGULATORY SUBUNITS FROM THE CULTURED RICE ( Oriza sativa) CELLS
• Cloning and Sequencing of cDNA from Oryza sativa encoding a homolog to non-ATPase subunit,MBP1, of 26S Proteasome in Arabidopsis thaliana
• PURIFICATION AND CHARACTERIZATION OF A 26S PROTEASOME FROM THE CULTURED RICE (Oriza sativa)CELLS
• Changes in Proteasome Levels in Spinach (Spinacia oleracea) Seeds during Imbibition and Germination
• Construction of novel vectors for transformation of Lentinula edodes using a chitin synthase gene promoter
• Purification, Characterization, and cDNA Cloning of a Novel α-Galactosidase from Mortierella vinacea
• Purification and Some Properties of α-Galactosidase from Penicillium purpurogenum
• Biological and Structural Properties of Cyclic Peptides Derived from the α-Amylase Inhibitor Tendamistat
• Synthesis and Properties of Cyclic Peptides Having Inhibitory Site of α-Amylase Inhibitor Tendamistat
• Influence of Day Length on Stem Growth, Flowering, Morphology of Flower Clusters, and Seed-Set in Buckwheat (Fagopyrum esculentum Moench)(Crop Physiology and Ecology)
• Influence of Day Length before and after the Start of Anthesis on the Growth, Flowering and Seed-Setting in Common Buckwheat (Fagopyrum esculentum Moench)
• Analysis of a Pressure-regulated Operon from the Barophilic Bacterium Strain DB6705
• Effect of high pressure on gene expression by lac and tac promoters in Escherichia coli
• Effects of Day Length and Air Temperature on Stem Growth and Flowering in Sesame(Crop Physiology & Ecology)
• Cloning of Lentinula edodes lemnp2, a manganese peroxidase that is secreted abundantly in sawdust medium
• Isolation of Industrial Strains of Aspergillus oryzae Lacking Ferrichrysin by Disruption of the dffA Gene(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Role of Hydroxylysine on the Nodulation of Root Nodule Bacteria
• Susceptibility to Hydrogen Peroxide and Catalase Activity of Root Nodule Bacteria
• Correlation between NaCl Sensitivity of Rhizobium Bacteria and Ineffective Nodulation of Leguminous Plants
• Effects of hydroxylysine on the growth and morphology of Rhizobium leguminosarum bv. phaseoli
• Inhibition of the Growth of Rhizobium Bacteria by Hydroxylysine
• Isolation and characterization of the gene encoding a manganese peroxidase from Lentinula edodes
• Cloning and Characterization of a Lentinula edodes Class II Chitin Synthase Gene, LeChs2
• Purification of a novel extracellular laccase from solid-state culture of the edible mushroom Lentinula edodes
• The Tyrosinase-Encoding Gene of Lentinula edodes, Letyr, Is Abundantly Expressed in the Gills of the Fruit-Body during Post-Harvest Preservation
• Isolation of Genes Specifically Expressed in the Fruit Body of the Edible Basidiomycete Lentinula edodes
• Mass Mating Method in Combination with G418- and Aureobasidin A-Resistance Markers for Efficient Selection of Hybrids from Homothallic Strains in Saccharomyces cerevisias
• A Simple Purification Method and Morphology and Component Analyses for Carotovoricin Er, a Phage-tail-like Bacteriocin from the Plant Pathogen Erwinia carotovora Er
• Isolation and Characterization of the Glyceraldehyde-3-phosphate Dehydrogenase Gene of Lentinus edodes
• Assembly of Staphylococcus aureus Leukocidin into a Pore-Forming Ring-Shaped Oligomer on Human Polymorphonuclear Leukocytes and Rabbit Erythrocytes
• Transformation of the Edible Basidiomycete Lentinus edodes by Restriction Enzyme-Mediated Integration of Plasmid DNA
• Purification and Properties of Tyrosinase Isozymes from the Gill of Lentinus edodes Fruiting Body
• Relationships between Tyrosinase Activity and Gill Browning during Preservation of Lentinus edodes Fruit-bodies
• Purification and Some Properties of Alanine Aminotransferase from Candida maltosa
• Production of _L-Tryptophan by 5-Fluorotryptophan and Indolmycin Resistant Mutants of Bacillus subtilis K(Microbiology & Fermentation Industry)
• Effect of L-Phenylalanine and L-Methionine on the Appearance of L-Tryptophan-Producing Mutants of Bacillus subtilis K
• Culture Conditions for Improvement of L-Threonine Production Using a Genetically Self-cloned L-Threonine Hyperproducing Strain of Escherichia coli K-12
• Construction of novel vectors for transformation of Lentinula edodes using a chitin synthase gene promoter(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Synthesis of Cyclic Peptides Derived from α-Amylase Inhibitor Tendamistat and Their Biological Activity
• Effective Recovery of Nd-Fe-B Sintered Magnet Scrap Powders as Microwave Absorbing Materials
• Effective Recovery of Nd-Fe-B Sintered Magnet Scrap Powders as Isotropic Bonded Magnets
• The Tyrosinase-Encoding Gene of Lentinula edodes, Letyr, Is Abundantly Expressed in the Gills of the Fruit-Body during Post-Harvest Preservation
• Cloning and Characterization of a Lentinula edodes Class II Chitin Synthase Gene, LeChs2
• dffA Gene from Aspergillus oryzae Encodes L-Ornithine N^5-Oxygenase and Is Indispensable for Deferriferrichrysin Biosynthesis(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• The Effect of the Amount of Nitrogen Fertilizer on Starch Metabolism in Leaf Sheath of Japonica and Indica Rice Varieties during the Heading Period(Crop Physiology and Ecology)
• Kinetic Studies on the Function of All the Conserved Tryptophans Involved Inside and Outside the QW Motifs of Squalene-hopene Cyclase : Stabilizing Effect of the Protein Structure against Thermal Denaturation
• Overexpression of Squalene-Hopene Cyclase by the pET Vector in Escherichia Coil and First Identification of Tryptophan and Aspartic Acid Residues inside the QW Motif as Active Sites
• Functional Analysis of the DXDDTA Motif in Squalene-Hopene Cyclase by Site-directed Mutagenesis Experiments : Initiation Site of the Polycyclization Reaction and Stabilization Site of the Carbocation Intermediate of the Initially Cyclized A-Ring
• Magnetic Properties of Nd-Fe-B Sintered Magnet Powders Sorbed by Yb Metal Vapor

もっと見る 閉じる

スポンサーリンク