dffA Gene from Aspergillus oryzae Encodes L-Ornithine N^5-Oxygenase and Is Indispensable for Deferriferrichrysin Biosynthesis(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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概要
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We identified and analyzed the dffA gene from Aspergillus oryzae which encodes L-ornithine N^5-oxygenase involved in the biosynthesis of deferriferrichrysin, a type of siderophore which is a low-molecular-weight iron chelating compound. From among more than 20,000 clones in an A. oryzae EST (expressed sequence tag) library, we found only one clone encoding a protein that exhibited homology to the Ustilago maydis sid1 protein (Sid1) and Pseudomonas aeruginosa pvdA protein (PvdA), both known as the only examples of L-ornithine N^5-oxygenase. The complete gene sequence shows that the dffA gene includes a 1575-bp open reading frame (ORF), one 66-bp intron, which is a typical intorn length in A. oryzae, and encodes 502 amino acids with putative FAD-binding, NADP-binding, and 'FATGY' motifs, which are conserved in N-hydroxylating enzymes. As well as that of the U. maydis sid1 gene, dffA gene expression was induced under iron-limited conditions, and the promoter region has several GATA-type transcription regulator binding motifs. When the dffA gene was expressed under the control of the α-amylase promoter in A. oryzae, transformants revealed inducible high L-ornithine N^5-oxygenase activities. In addition, a dffA gene disruptant showed no deferriferrichrysin production even under iron-limited conditions. These results clearly suggest that the dffA gene is indispensable for deferriferrichrysin biosynthesis in A. oryzae.
- 公益社団法人日本生物工学会の論文
- 2003-01-25
著者
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Akao Takeshi
National Research Institute of Brewing
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Yamada Osamu
National Cardiovascular Center
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Enei Hitoshi
Iwate Biotechnology Research Center
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Watanabe Hisayuki
Iwate Biotechnology Research Center
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Akita O
Fac. Of Human Life Sciences Dep. Of Food And Health Sciences Jissen Women's Univ.
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Akita Osamu
National Research Institute Of Brewing
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Yamato Osamu
Kagoshima Univ. Kagoshima
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Akao T
National Research Institute Of Brewing
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Yamada O
Laboratory Of Clinical Pathology Department Of Veterinary Clinical Sciences Faculty Of Agriculture K
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TOMINAGA Mihoko
National Research Institute of Brewing
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NAN SUTHAMAS
National Research Institute of Brewing
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SATOH TOSHITSUGU
Iwate Biotechnology Research Center
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Watanabe Hisayuki
Life Environment Medical Engineering Division Of Medical And Engineering Science Interdisciplinary G
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Nan Suthamas
National Research Institute Of Brewing:(present Address)plant Pathology And Microbiology Division De
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