Gene Silencing by RNA Interference in the Koji Mold Aspergillus oryzae

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概要

• 論文の詳細を見る

• We found the orthologous genes required for RNA interference (RNAi) in the Aspergillus oryzae genome database, and constructed a set of tools for gene silencing using RNAi in A. oryzae. This system utilizes compatible restriction enzyme sites so that only a single target gene fragment is required to create the hairpin RNA cassette. For ease of handling, we also separated the construction of the hairpin RNA cassette for the target gene from its subsequent introduction into the expression vector. Using the brlA gene as a target for RNAi, we detected decreased mRNA levels and a delayed conidiation phenotype in the transformants. Furthermore, even though A. oryzae possesses three copies of the α-amylase gene, a single copy of an α-amylase RNAi construct was sufficient to downregulate the mRNA levels and decrease the enzymatic activity to 10% of control levels. Gene silencing by RNAi should provide a powerful genetic tool for post-genomic studies of the industrially important fungus A. oryzae.

• 社団法人 日本農芸化学会の論文
• 2007-01-23

著者

• Yamada Osamu

  National Cardiovascular Center

• Sakamoto K

  Fermentation Research Laboratories Fujisawa Pharmaceutical Co. Ltd.

• Sakamoto Kazutoshi

  National Research Institute Of Brewing

• Akita O

  Fac. Of Human Life Sciences Dep. Of Food And Health Sciences Jissen Women's Univ.

• Akita Osamu

  National Research Institute Of Brewing

• Yamato Osamu

  Kagoshima Univ. Kagoshima

• Yamada O

  Laboratory Of Clinical Pathology Department Of Veterinary Clinical Sciences Faculty Of Agriculture K

• Hayashi Risa

  National Research Institute Of Brewing

• IKEDA Ryoko

  National Research Institute of Brewing

• OHKITA Yuka

  National Research Institute of Brewing

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