A Novel Amine Oxidase-Encoding Gene from Aspergillus oryzae(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
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概要
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We cloned a novel gene (aoxA) encoding amine oxidase (AOX) from Aspergillus oryzae. One cDNA clone showing extreme homology to the AOX-encoding genes was found in an expressed sequence tag (EST) library of A. oryzae. Molecular analysis revealed that the aoxA carried four exons interrupted by three introns and had an open reading frame encoding 672 amino acid residues. The deduced amino acid sequence showed about 83.5% identity to the Aspergillus niger AO-I. The strictly conserved residues for co-factor and copper binding in copper/quinine-containing AOXs were also preserved at Tyr 405, His 456, His 458 and His 617 in the cDNA sequence. When the aoxA was overexpressed in the homologous hyperexpression system of A. oryzae, AOX activity in the transformant was enhanced 75-fold. An apparent molecular weight of 159,000 by gel filtration and a subunit molecular weight of 75,000 by SDS-PAGE of the purified enzyme were estimated, suggesting that the enzyme molecule is a homo-dimer similar to other copper/quinine- containing AOXs. The A. oryzae AOXA preferentially oxidized aliphatic monoamines of C2-C6 rather than aromatic amines or diamines. From these results, the aoxA gene product obtained by homologous hyperexpression system of A. oryzae is undoubtedly a functional AOX.
- 社団法人日本生物工学会の論文
- 2004-11-25
著者
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Hata Yoji
Research Institute, Gekkeikan Sake Co. Ltd.
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Hata Yoji
Res. Inst. Gekkeikan Sake Co. Ltd. 101 Shimotoba-koyanagi-cho Fushimi-ku Kyoto 612-8385 Jpn
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Abe Yasuhisa
Res. Inst. Gekkeikan Sake Co. Ltd.
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Abe Yasuhisa
Research Institute For Fundamental Physics Kyoto University : Centre De Recherches Nucleaires
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Abe Yasuhisa
Research Institute For Fundamental Physics Kyoto University
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MATSUMURA Kengo
Research Institute, Gekkeikan Sake Co., Ltd.
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KAWATO Akitsugu
Research Institute, Gekkeikan Sake Co., Ltd.
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AKITA Osamu
National Research Institute of Brewing (NRIB)
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Obata Hiroshi
Research Institute Gekkeikan Sake Co. Ltd.
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HISADA HIROMOTO
Research Institute, Gekkeikan Sake Co. Ltd.
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Hata Yoji
Research Institite Of Gekkeikan Gekkeikan Sake Co. Ltd.
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Abe Yasushisa
Research Institite Of Gekkeikan Gekkeikan Sake Co. Ltd.
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Akita O
Fac. Of Human Life Sciences Dep. Of Food And Health Sciences Jissen Women's Univ.
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Akita Osamu
National Research Institute Of Brewing
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Kawato A
Research Institute Gekkeikan Sake Co. Ltd.
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Kawato Akitsugu
Research Institute Gekkeikan Sake Co. Ltd.
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Hisada Hiromoto
Research Institute Gekkeikan Sake Co. Ltd.
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Abe Yasuhisa
Research Institute Gekkeikan Sake Co. Ltd.
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Matsumura Kengo
Research Institute Gekkeikan Sake Co Ltd.
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Abe Yasuhisa
Research Center For Nuclear Physics Osaka University
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