Direct Ethanol Production from Barley β-Glucan by Sake Yeast Displaying Aspergillus oryzae β-Glucosidase and Endoglucanase(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
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概要
- 論文の詳細を見る
Three β-glucosidase- and two endoglucanase-encoding genes were cloned from Aspergillm oryzae, and their gene products were displayed on the cell surface of the sake yeast, Saccharomyces cerevislae GRI-117-UK. GRI-117-UK/pUDB7 displaying β-glucosidase AO090009000356 showed the highest activity against various substrates and efficiently produced ethanol from cellobiose. On the other hand, GRI-117-UK/pUDCB displaying endoglucanase AO090010000314 efficiently degraded barley β-glucan to glucose and smaller cellooligosaccharides. GRI-117-UK/pUDB7CB codisplaying both β-glucosidase AO090009000356 and endoglucanase AO090010000314 was constructed. When direct ethanol fermentation from 20 g/l barley β-glucan as a model substrate was performed with the codisplaying strain, the ethanol concentration reached 7.94 g/l after 24 h of fermentation. The conversion ratio of ethanol from β-glucan was 69.6% of the theoretical ethanol concentration produced from 20 g/l barley β-glucan. These results showed that sake yeast displaying A. oryzae cellulolytic enzymes can be used to produce ethanol from cellulosic materials. Our constructs have higher ethanol production potential than the laboratory constructs previously reported.
- 2008-06-25
著者
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Kotaka Atsushi
Research Institute, Gekkeikan Sake Co. Ltd.
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Sahara Hiroshi
Research Institute, Gekkeikan Sake Co. Ltd.
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Kuroda Kouichi
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Kondo Akihiko
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University
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Ueda Mitsuyoshi
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Hata Yoji
Research Institute, Gekkeikan Sake Co. Ltd.
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Hata Yoji
Research Institute Gekkeikan Sake Co. Ltd.
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Kuroda Kouichi
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Sahara Hiroshi
Research Institute Gekkeikan Sake Co. Ltd.
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Kotaka Atsushi
Research Institute Gekkeikan Sake Co. Ltd.
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KAYA Masahiko
Research Institute, Gekkeikan Sake Co., Ltd.
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Kondo Akihiko
Department Of Bioactive Molecules National Institute Of Infectious Diseases
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Hata Yoji
Research Institite Of Gekkeikan Gekkeikan Sake Co. Ltd.
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Kaya Masahiko
Research Institute Gekkeikan Sake Co. Ltd.
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Bando Hiroki
Research Institute, Gekkeikan Sake Co. Ltd.
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Kato-Murai Michiko
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Bando Hiroki
Research Institute Gekkeikan Sake Co. Ltd.
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Ueda Mitsuyoshi
Division Of Applied Life Science Graduate School Of Agriculture Kyoto University
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Kuroda Kouichi
Division Of Applied Life Science Graduate School Of Agriculture Kyoto University
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Kato-murai Michiko
Research Institute Gekkeikan Sake Co. Ltd.
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Kato-murai Michiko
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Kondo Akihiko
Department Of Applied Chemistry Kyushu Institute Of Technology
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Kondo Akihiko
Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology
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