Comparision of Two Glucoamylases Produced by Aspergillus oryzae in Solid-State Culture (Koji) and in Submerged Culture
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概要
- 論文の詳細を見る
Two extracellular glucoamylases (EC 3.2.1.3) of Aspergillus oryzae were purified from solid-state culture (S-GA) and from submerged culture (L-GA). The two glucoamylases have differnt molecular masses, 65 kDa for L-GA; 63-99 kDa for S-GA, and different isoelectric points, 4.2 for L-GA; 3.9 for S-GA. Almost all of the enzymatic characteristics of the two glucoamylases were similar, except for thermal stability, initial reaction velocity on pullulan and K_m value with soluble starch. Although L-GA could digest raw starch, S-GA demonstrated little activity with raw starch. Peptide mapping and amino acid composition showed that L-GA must be encoded by the glaA gene previously cloned as the glucoamylase-encoding gene from A. oryzae, but S-GA had a different primary structure than the deduced glaA product. Introduction of multiple copies of the glaA gene to A. oryzae caused on elevation of glucoamylase productivity of transformant in submerged culture but not in solid-state culture. These results suggested that the two forms of glucoamylases arise from different genes rather than result from proteolytic processing after polypeptide synthesis of a single protein.
- 社団法人日本生物工学会の論文
- 1997-12-25
著者
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Hata Yoji
Research Institute, Gekkeikan Sake Co. Ltd.
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Hata Yoji
Res. Inst. Gekkeikan Sake Co. Ltd. 101 Shimotoba-koyanagi-cho Fushimi-ku Kyoto 612-8385 Jpn
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Ishida H
Research Institute Gekkeikan Sake Co. Ltd.
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Ishida Hiroki
Research Institute Gekkeikan Sake Co. Ltd.
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KAWATO Akitsugu
Research Institute, Gekkeikan Sake Co., Ltd.
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ICHISHIMA EIJI
Department of Bioengineering, Graduate School of Engineering, Soka University
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Ichishima Eiji
Department Of Bioengineering Graduate School Of Engineering Soka University
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Ichishima E
Department Of Bioengineering Graduate School Of Engineering Soka University
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Hata Yoji
Research Institite Of Gekkeikan Gekkeikan Sake Co. Ltd.
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IMAYASU Satoshi
Research Institite of Gekkeikan, Gekkeikan Sake Co., Ltd.
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SUGINAMI Koji
Research Institute, Gekkeikan Sake Co. Ltd.
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Yasui Katsuhiro
Research Institute Gekkeikan Sake Co. Ltd.
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Kawato A
Research Institute Gekkeikan Sake Co. Ltd.
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Kawato Akitsugu
Research Institute Gekkeikan Sake Co. Ltd.
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Imayasu Satoshi
Research Institute Gekkeikan Sake Co. Ltd.
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ICHIKAWA EIJI
Research Institute, Gekkeikan Sake Co.Ltd.
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KOJIMA YASUHIRO
Research Institute, Gekkeikan Sake Co. Ltd.,
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Suginami K
Research Institute Gekkeikan Sake Co. Ltd.
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Suginami Koji
Research Institute Gekkeikan Sake Co. Ltd.
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Ichikawa Eiji
Gekkeikan Research Institute Gekkeikan Sake Co. Ltd.
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