Deletion Analysis of the Catalase-Encoding Gene (catB) Promoter from Aspergillus oryzae
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概要
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The catalase-encoding gene (catB) is expressed strongly in Aspergillus oryzae. To identify the transcription regulatory elements involved in strong expression, we did promoter deletion analysis using β-glucuronidase (GUS) as a reporter and an electrophoretic gel mobility shift assay (EMSA) systematically. The deletion 200-bp sequence from −1,000 to −800 in the 1,400-bp catB promoter caused a drastic decrease in GUS activity. In addition, EMSA implicated a 45-bp element from −1,000 to −956 containing cis-elements. According to detailed promoter deletion analysis, a region from −1,000 to −975, which contains putative heat shock element (HSE) and the CCAAT-box, was involved in strong expression.
- 社団法人 日本農芸化学会の論文
- 2008-01-23
著者
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Sano Motoaki
Gene Regulation Group Molecular And Cell Biology National Inst. Of Advanced Industrial Sci. And Tech
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Hata Yoji
Res. Inst. Gekkeikan Sake Co. Ltd. 101 Shimotoba-koyanagi-cho Fushimi-ku Kyoto 612-8385 Jpn
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Abe Yasuhisa
Research Institute For Fundamental Physics Kyoto University
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Hata Yoji
Research Institite Of Gekkeikan Gekkeikan Sake Co. Ltd.
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Machida Masayuki
Gene Regulation Group Molecular And Cell Biology National Institute Of Advanced Industrial Science A
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Hisada Hiromoto
Research Institute Gekkeikan Sake Co. Ltd.
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Abe Yasuhisa
Research Institute Gekkeikan Sake Co. Ltd.
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Machida Masayuki
Gene Regulation Group Molecular And Cell Biology National Institute Of Advanced Industrial Science A
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Abe Yasuhisa
Research Center For Nuclear Physics Osaka University
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