Glycosylated DNA-Binding Proteins from Filamentous Fungus, Aspergillus oryzae : Modification with N-Acetylglucosamine Monosaccharide through an O-Glycosidic Linkage
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概要
- 論文の詳細を見る
The existence of glycosylated DNA-binding proteins was demonstrated in a whole cell extract from a filamentous fungus, Aspergillus oryzae. The proteins were specifically eluted from a DNA-cellulose column by the eluate containing shared double-stranded DNA and were detected by wheat germ agglutinin (WGA)-probing. The apparent molecular masses of these proteins on SDS-PAGE were 140 kDa, 115 kDa, 105 kDa, 68 kDa, and 60 kDa. The labeling of the proteins by uridine 5'-diphosphate(UDP)-[^<14>C]galactose using galactosyltransferase showed the same electrophoretic pattern with the WGA-probing. The [^<14>C]-galactose-labeled saccharides were released from the proteins by mild-base treatment but not by N-glyco-peptidase F digestion, indicating the O-glycosidic linkage of the saccharide chain attachment to proteins. The [^<14>C]galactose-labeled saccharides co-migrated with galactose-(β1→4)-N-acetylglucosaminitol on a silica gel plate. Thus, it was seen that several proteins which had the DNA-binding activity were modified by N-acetylglucosamine monosaccharide through an O-glycosidic linkage in A. oryzae.
- 社団法人日本農芸化学会の論文
- 1994-02-23
著者
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Machida Masayuki
Molecular Biology Department National Institute Of Bioscience And Human-technology
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Machida M
Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A
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Jigami Yoshifumi
Molecular Biology Department, National Institute of Bioscience and Human-Technology
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Jigami Yoshifumi
Molecular Biology Department National Institute Of Bioscience And Human-technology
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Machida Masayuki
Gene Regulation Group Molecular And Cell Biology National Institute Of Advanced Industrial Science A
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