Construction of a Positive Selection Marker by a Lethal Gene with the Amber Stop Codon(s) Regulator
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概要
- 論文の詳細を見る
A novel positive selection marker for Escherichia coli transformation was developed. The marker consisted of a DNA fragment encoding the C-terminal ribonuclease domain (CRD) of colicin E3 (colE3) and one or more amber stop codons between the initiation codon and the E3-CRD coding sequence. The toxicity of the marker was controlled by the suppressor activity the host cells possessed. This allowed both effective selection and propagation of the vector possessing the maker by selecting appropriate hosts from among those widely distributed: sup+ strains for selection and sup0 strains for propagation respectively. The insert DNA fragment was introduced onto the vector by replacing the marker DNA. The transformants harboring the vector with an insert grew, but those without an insert were effectively removed by the killing activity of E3-CRD encoded on the marker DNA. The marker was also successfully applied to λ phage display vector.
- 社団法人 日本農芸化学会の論文
- 2006-01-23
著者
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Yohda Masafumi
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
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MASAKI Haruhiko
Department of Biotechnology, The University of Tokyo
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Yohda Masafumi
Department Of Biotechnology And Life Science Graduate School Of Engineering Tokyo University Of Agri
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Masaki Haruhiko
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Kunihiro Sumiko
Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A
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Machida Masayuki
Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A
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Machida M
Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A
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Masaki H
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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OHASHI KUNIHIRO
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science
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HAGIWARA Hiroko
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science
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Hagiwara Hiroko
Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A
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Machida Masayuki
Gene Regulation Group Molecular And Cell Biology National Institute Of Advanced Industrial Science A
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Ohashi-Kunihiro Sumiko
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST)
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