Mutational Analysis of the Potato Virus Y 5' Untranslated Region for Alteration in Translational Enhancement in Tobacco Protoplasts
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概要
- 論文の詳細を見る
The 185 nucleotide 5' untranslated region (5'UTR) of potato virus Y ordinary strain (PVY-O) showed translation-enhancing activity on the β-glucuronidase (GUS) gene in tobacco protoplasts. Mutational analysis of the 5'UTR was done to find sequence motifs necessary for the enhancement. Deletions within the 1-130 nucleotide region of 5'UTR stimulated the GUS expression in some cases, while the GUS activity declined with deletions in the 131-185 nucleotide region. The results indicated that the last 55 nucleotides of PVY-O 5'UTR might play the much important role in the translational enhancement in plant cells.
- 社団法人日本農芸化学会の論文
- 1997-12-23
著者
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HIDAKA Makoto
Department of Biotechnology, The University of Tokyo
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MASAKI Haruhiko
Department of Biotechnology, The University of Tokyo
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Hidaka Makoto
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Yang L
Department Of Biotechnology Graduate School Of Agicultural And Life Sciences The University Of Tokyo
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Yang Li
Department Of Obstetrics And Gynecology Miyazaki Medical College
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SONODA Junichiro
Department of Clinical Pharmacology, Faculty of Medicine, Kagoshima University
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Uozumi T
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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UOZUMI Takeshi
Department of Biotechnology, Faculty of Agriculture, The University of Tokyo
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Masaki Haruhiko
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Sonoda Junichiro
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Uozumi Takeshi
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Uozumi Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Masaki H
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Yang Li
Department Of Biotechnology Graduate School Of Agicultural And Life Sciences The University Of Tokyo
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Yang Li
Department of Bioengineering, Chongqing University
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