Construction of New Shuttle Vectors for Acetobacter
スポンサーリンク
概要
- 論文の詳細を見る
Shuttle vector pMV301 was constructed by ligation of pMV102 found in A. aceti subsp. xylinum NBI 1002 to E. coli plasmid pACYC177. It is 6.0kb in size, has unique restriction sites suitable for insertion of a foreign DNA fragment and confers ampicillin resistance to the Acetobacter host. This vector transforms A. aceti subsp. aceti 10-80S1 and industrial vinegar producer A. aceti subsp. xylinum NBI 1002 as well as E. coli. Various chimeric plasmids were also constructed by ligation of pMV102 to E. coli plasmids to examine the expression of drug resistance genes. In addition to the ampicillin resistance gene, resistance genes for kanamycin, chloramphenicol and tetracycline derived from E. coli plasmids were expressed in Acetobacter. Most of the constructed shuttle vectors were stably maintained in Acetobacter.
- 社団法人 日本農芸化学会の論文
著者
-
OKUMURA HAJIME
Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd.,
-
Uozumi Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
-
Masai Hiroshi
Nakano Biochemical Research Institute Nakano Vinegar Co. Ltd.
-
Beppu Teruhiko
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
-
FUKAYA Masahiro
Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd.
-
OKUMURA Hajime
Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd.
関連論文
- Enhancement of Acetic Acid Production in a High Cell-Density Culture of Acetobacter aceti
- Effects of Dissolved Oxygen and Acetic Acid Concentrations on Acetic Acid Production in Continuous Culture of Acetobacter aceti
- Radicicol Binding to Swo1/Hsp90 and Inhibition of Growth of Specific Temperature-sensitive Cell Cycle Mutants of Fission Yeast(Biochemistry & Molecular Biology)
- Identification of Preussin as a Selective Inhibitor for Cell Growth of the Fission Yeast ts Mutants Defective in Cdc2-Regulatory Genes
- Morphology of Ras- Transformed Cells Becomes Apparently Normal Again with Tyrosine Kinase Inhibitors without a Decrease in the Ras- GTP Complex^1
- Basidifferquinone Analogues, Basidifferquinone B and C, Which Induce Fruiting-body Formation of a Basidiomycete, Favolus arcularius
- Radicicol, an Agent Inducing the Reversal of Transformed Phenotypes of src-Transformed Fibroblasts
- Basidifferquinone, a New Inducer for Fruiting-body Formation of a Basidiomycetes Favolus arcularius from a Streptomyces Strain II. Structure of Basidifferquinone(Organic Chemistry)
- Basidifferquinone, a New Inducer for Fruiting-body Formation of a Basidiomycetes Favolus arcularius from a Streptomyces Strain I. Screening and Isolation(Organic Chemistry)
- Components of the Cytochrome P-450 Monooxygenase System of the Fungus, Fusarium oxysporum : Conditions for Induction and Isolation of Cytochrome b_5(Biological Chemistry)
- Expression of Differentiation-related Markers in Teratocarcinoma Cells via Histone Hyperacetylation by Trichostatin A(Biological Chemistry)
- Complete Amino Acid Sequence of Crystalline α-Glucosidase from Aspergillus niger
- Intraspecific Hybridization by Protoplast Fusion in Mucorales Producing Milk-clotting Proteases(Microbiology & Fermentation Industry)
- Detection of Potato Virus Y P1 Protein in Infected Cells and Analysis of Its Cleavage Site
- Mutational Analysis of the Potato Virus Y 5' Untranslated Region for Alteration in Translational Enhancement in Tobacco Protoplasts
- Addition of a Small Amount of an Endoglucanase Enhances Cellulose Production by A cetobacter xylinum
- Establishing the Independent Culture of a Strictly Symbiotic Bacterium Symbiobacterium thermophilum from Its Supporting Bacillus Strain
- Nitrile Hydratase Gene from Rhodococcus sp. N-774 Requirement for Its Downstream Region for Efficient Expression
- Identification of the Cellulose-Binding Domain of a Bacillus subtilis Endoglucanase Distinct from Its Catalytic Domain
- Characterization of the Leader Peptide of an Endo-type Cellulase Produced by an Alkalophilic Streptomyces Strain(Microbiology & Fermentation Industry)
- Purification and Properties of Thermostable β-Tyrosinase from an Obligately Symbiotic Thermophile, Symbiobacterium thermophilum
- Purification and Properties of Thermostable Tryptophanase from an Obligately Symbiotic Thermophile, Symbiobacterium thermophilum(Microbiology & Fermentation Industry)
- Replacements of Amino Acid Residues at Subsites and Their Effects on the Catalytic Properties of Rhizomucor pusillus Pepsin, an Aspartic Proteinase from Rhizomucor pusillus
- Site-Directed Mutagenesis of Conserved Trp39 in Rhizomucor pusillus Pepsin: Possible Role of Trp39 in Maintaining Tyr75 in the Correct Orientation for Maximizing Catalytic Activity
- Cloning of a New cryIA(a) Gene from Bacillus thuringiensis Strain FU-2-7 and Analysis of Chimaeric CryIA(a) Proteins for Toxicity
- Involvement of AfsA in A-factor Biosynthesis as a Key Enzyme
- Characterization of the Biosynthetic Pathway of Cellulose from Glucose and Fructose in Acetobacter xylinum
- Transcriptional Regulation of the Bacillus ohbensis Cyclodextrin Glucanotransferase Gene in B. subtilis
- Isolation and Characterization of the Actin Gene from the Cellulolytic Fungus Humicola grisea and Analysis of Transcription Levels of Actin and Cellulase Genes
- Molecilar Clpmomg amd Expression of the Novel Fungal β-Glucsidase Gennes from Humicola grisea and Trichoderma reesei^1
- Isolation of the creA Gene from the Cellulolytic Fungus Humicola grisea and Analysis of CreA Binding Sites Upstream from the Cellulase Genes
- Characterization of Mutants Defective in Melanogenesis and a Gene for Tyrosinase of Streptomyces griseus
- Two Genes Encoding Serine Protease Homologues in Serratia marcescens and Characterization of Their Products in Escherichia coli^1
- Transformation of Acetobacter polyoxogenes with Plasmid DNA by Electroporation
- The aarC Gene Responsible for Acetic Acid Assimilation Confers Acetic Acid Resistance on Acetobacter aceti
- Spheroplast Fusion of Acetobacter aceti and Its Application to the Breeding of Strains for Vinegar Production(Microbiology & Fermentation Industry)
- Specific Excretion into the Medium of a Serine Protease from Serratia marcescens(Microbiology & Fermentation Industry)
- Characterization and Distribution of IS1164 that Exists in the High Molecular Mass Nitrile Hydratase Gene Cluster of the Industrial Microbe Rhodococcus rhodochrous J1
- Cloning of the β-Isopropylmalate Dehydrogenase Gene from Acetobacter aceti and Its Use for Construction of a New Host-vector System for Acetobacter(Microbiology & Fermentation Industry)
- Isolation of the Gene and Characterization of the Enzymatic Properties of a Major Exoglucanase of Humicola grisea without a Cellulose-Binding Domain
- Oxygen Sensitivity of NifA Protein of Azospirillum lipoferum FS as Suggested by Gene Cloning and Expression in Escherichia coli
- Mutational Analysis of the Amino Acid Residues Essential for the cis and trans Cleavage Activity of the Potato Virus Y 50-kDa Protease
- Cleavage of Potato Virus Y Polyprotein in Escherichia coli Depending on the Proteolytic Activity of Viral Protease
- NifA Protein Synthesized In Vitro Binds to the Upstream Activator Sequence in the Promoter of the Klebsiella oxytoca nifB Gene(Microbiology & Fermentation Industry)
- Major Anthocyanin of the Flowers of Hibiscus (Hibiscus rosa-sinensis L.)(Organic Chemistry)
- A Host-Vector System for a Cellulose-Producing Acetobacter Strain
- Enhanced Production of Antibiotics by Pecilomyces lilacinus under Alkaline Conditions Associated with Morphological Change(Microbiology & Fermentation Industry)
- Cloning of a Gene Encoding a Putative Xylanase with a Cellulose-Binding Domain from Humicola grisea
- Cloning, Sequencing, and Expression of a Thermostable Cellulase Gene of Humicola grisea
- Enhanced Expression of cryIA(a) Gene of Bacillus thuringiensis in Escherichia coli
- Cloning of a Gene Encoding a Putative Carbon Catabolite Repressor from Trichodermareesei
- Purification and Characterization of Cellulases from Humicola grisea
- Extracellular Production of Bacillus ohbensis Cyclodextrin Glucanotransferase by B. subtilis
- Use of a Triple Protease-deficient Mutant of Bacillus subtilis as a Host for Secretion of a B. subtilis Cellulase and TEM β-Lactamase(Microbiology & Fermentation Industry)
- Purification and Properties of Cellulases from an Alkalophilic Streptomyces Strain(Microbiology & Fermentation Industry)
- Cloning and Expression of the nifA Gene of Klebsiella oxytoca in K. pneumoniae and Azospirillum lipoferum(Microbiology & Fermentation Industry)
- Metabolism of α-Hydroxyglutarate by an Alcaligenes sp.(Biological Chemistry)
- Isolation and Characterization of a Protease-Producing, Thermophilic, Anaerobic Bacterium, Thermobacteroides leptospartum sp. nov.(Microbiology & Fermentation Industry)
- Signal Peptide of the Colicin E2 Lysis Protein Causes Host Cell Death(Microbiology & Fermentation Industry)
- Streptomyces Species as Boundary Microorganisms : Eucaryotic Regulatory Systems for Secondary Metabolism and Morphological Differentiation
- Overexpression of a Gene Cluster Encoding a Chalcone Synthase-like Protein Confers Redbrown Pigment Production in Streptomyces griseus
- Secretion by Saccharomyces cerevisiae of Human Apolipoprotein E as a Fusion to Serum Albumin
- High Level Secretion by Saccharomyces cerevisiae of Human Apolipoprotein E as a Fusion to Rhizomucor Rennin
- Suppression of an Ethanol-Sensitive Mutation of Acetobacter pasteurianus by Overexpression of the his1 Gene Encoding Histidinol Phosphate Aminotransferase
- Characterization of Nitrile Hydratase Genes Cloned by DNA Screeing from Rhodococcus erythropolis
- Structure of an Acidic Polysaccharide Elaborated by Acetobacter sp. NBI 1005(Biological Chemistry)
- Construction of a Bacillus subtilis Strain Deficient in Three Proteases(Biological Chemistry)
- Heat-stable Amylase Complex Produced by a Strictly Anaerobic and Extremely Thermophilic Bacterium, Dictyoglomus thermophilum(Microbiology & Fermentation Industry)
- Stimulatory Effect of Copper on Antibiotic Production and Morphological Differentiation in Streptomyces tanashiensis
- Involvement of a Small ORF Downstream of the afsR Gene in the Regulation of Secondary Metabolism in Streptomyces coelicolor A3(2)
- Wide Distribution of Interspecific Stimulatory Events on Antibiotic Production and Sporulation Among Streptomyces species
- Studies on Antibiotics Produced at High Alkaline pH
- Effects of A New Polysaccharide (AM-1) Produced by Acetobacter sp. on Glucose Tolerance in Rats
- Development of a Host-vector System for Gluconobacter suboxydans
- Isolation and Characterization of a New Polysaccharide-producing Acetobacter sp.
- Rapid Purification and Crystallization of Thermostable Malate Dehydrogenase Isolated from a Thermophilic Bacterium, Thermus flavus AT 62
- Extensive Purification and Characterization of L-Alloisocitrate Dehydrogenase and Oxalosuccinate Decarboxylase of a Pseudomonad
- Biochemical Characteristics of Spontaneous Mutants of Acetobacter aceti Deficient in Ethanol Oxidation
- Improved Direct Expression of Prochymosin cDNA through Changing the SD-ATG Codon Length
- Molecular Cloning of Thermus flavus Malate Dehydrogenase Gene
- Distribution and Characterization of Plasmids in Acetic Acid Bacteria
- Production of Chymosin in Escherichia coli Cells and Its Enzymatic Properties
- Intraspecific and Interspecific Hybridization of Mucor pusillus and M. miehei by Protoplast Fusion
- Structure of an Acidic Polysaccharide from Acetobacter sp. NBI 1022
- Improved Transformation Method for Acetobacter with Plasmid DNA
- Construction of New Shuttle Vectors for Acetobacter
- Cloning and Expression in E. coli of the Whole nif Genes of Klebsiella oxytoca, a Nitrogen Fixer in the Rhizosphere of Rice
- Molecular Cloning of a Cellulase Gene from Bacillus subtills and Its Expression in Escherichia coli
- Construction of a Safer and Useful Host for Molecular Cloning in Bacillus subtilis
- Cloning and Expression of a Gene for Resistance to Butirosin both in Escherichia coli and Bacillus subtilis
- Regulation of Nitrite Reductase in the Denitrifying Bacterium Alcaligenes faecalis S-6
- Screening of Antifungal Antibiotics According to Activities Inducing Morphological Abnormalities
- Membrane Filter Method for Isolation and Characterization of Thermophilic Bacteria
- Trichostatin C, a New Inducer of Differentiation of Friend Leukemic Cells
- Hygromycin A and Methoxyhygromycin, Novel Inhibitors of K88 Antigen Synthesis of Enterotoxic Escherichia coli Strain