Cloning and Expression of a Gene for Resistance to Butirosin both in Escherichia coli and Bacillus subtilis
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概要
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We have cloned a butirosin resistance gene located on a 2.9Md HindIII fragment of the chromosomal DNA of Bacillus vitellinus Z-1159, a butirosin producer, on a shuttle vector, pTA1302 (5.2Md, Apr, Cmr). The recombinant plasmid conferred resistances to butirosin, neomycin and kanamycin, on both Escherichia coli and Bacillus subtilis. Functional expression of aminoglycoside-B -phosphotransferase activity from the butirosin resistance gene was confirmed in the cell extracts of both E. coli and B. subtilis harboring the recombinant plasmid. A cleavage map of the recombinant plasmid was constructed.
- 社団法人 日本農芸化学会の論文
著者
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Uozumi Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Beppu Teruhiko
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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HOSHINO Takayuki
Department of Agricultural Chemistry, The University of Tokyo
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