Components of the Cytochrome P-450 Monooxygenase System of the Fungus, Fusarium oxysporum : Conditions for Induction and Isolation of Cytochrome b_5(Biological Chemistry)
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概要
- 論文の詳細を見る
Not only Cytochrome P-450 but also cytochrome b_5-like hemeproteins, NADPH-cytochrome c reductase, and NADH-ferricyanide reductase activities could be detected in the cell-free extract of a Cytochrome P-450-producing fungus, Fusarium oxysporum. Both cytochromes (P-450 and b_5) could be reduced anaerobically with a natural reductant (NADPH or NADH), suggesting the existence of an electron transport system to reduce the cytochromes. Further, two monooxygenase activities, i.e., N-demethylase and benzo[a]pyrene monooxygenase, were also found in the extract in addition to the fatty acid hydroxylase previously found. These components and activities seemed to be induced concomitantly. One of the cytochrome b_5-like hemeproteins was isolated, the absorption spectra and molecular weight (M_r=16,200) of which closely resembled those of hepatic cytochrome b_5 of mammals. From these results we concluded that this fungus has a cytochrome P-450-containing electron transport system similar to that in hepatic microsomes of mammals in addition to the unique nitrate/nitrite-inducible cytochrome P-450 system previously found.
- 社団法人日本農芸化学会の論文
- 1989-08-23
著者
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SHOUN Hirofumi
Department of Biotechnology, The University of Tokyo
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Shoun Hirofumi
Department Of Agricultural Chemistry The University Of Tokyo:(present Office)institute Of Applied Bi
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BEPPU Teruhiko
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tok
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SUDO Yoshiaki
Department of Agricultural Chemistry, The University of Tokyo
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SATO Ken-ichi
Department of Agricultural Chemistry, The University of Tokyo
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Sudo Yoshiaki
Department Of Agricultural Chemistry The University Of Tokyo:(present Office)sumitomo Pharmaceutical
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Sato Ken-ichi
Department Of Agricultural Chemistry The University Of Tokyo:(present Office)nihon Pall Ltd.
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Beppu Teruhiko
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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