Transformation of Acetobacter polyoxogenes with Plasmid DNA by Electroporation

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概要

• 論文の詳細を見る

• Acetobacter polyoxogenes was transformed with plasmid DNA by electroporation. The following points were essential for transformation: (i) dilution of the culture broth with cold water and air bubbling of the culture broth for transformation at discharging from a jar fermentor, and (ii) selection of transformants by liquid cultivation. For shortening of the lag time in cultivation for selection of transformants, the following treatments were useful: (i) addition of sucrose to the cell suspension during transformation and to the broth for cultivation, and (ii) addition of 1mM MgCl_2 to a mixture of cells and DNA during electroporation.

• 社団法人日本農芸化学会の論文
• 1994-05-23

著者

• Ohtake H

  Institute Of Applied Microbiology University Of Tokyo

• Ohtake Hisao

  Department Of Fermentation Technology Hiroshima University

• FUKAYA MASAHIRO

  Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd.,

• OKUMURA HAJIME

  Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd.,

• KAWAMURA YOSHIYA

  Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd.,

• HORINOUCHI Sueharu

  Department of Biotechnology, University of Tokyo

• Fukuwaka Masa-aki

  Hokkaido National Fisheries Research Institute

• Toda K

  Univ. Tokyo Tokyo Jpn

• Toda K

  Department Of Applied Chemistry And Biotechnology Niigata Institute Of Technology

• Toda K

  Faculty Of Engineering Niigata Institute Of Technology

• BEPPU Teruhiko

  Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tok

• Tayama Kenji

  Nakano Biochemical Research Institute Nakano Vinegar Co. Ltd.

• Horinouchi Sueharu

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Horinouchi Sueharu

  Dep. Of Biotechnology Graduate School Of Agriculture And Life Sciences Univ. Of Tokyo

• Beppu T

  Department Of Applied Biological Science Nihon University

• Toda Kiyoshi

  Faculty Of Engineering Niigata Institute Of Technology

• Kawamura Y

  National Food Research Inst. Tsukuba Jpn

• Kawamura Y

  Tsuruga Institute Of Biotecnology Tokyo Co. Ltd.

• Fukaya M

  Central Research Institute Mitsukan Group Corporation

• Beppu Teruhiko

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• FUKAYA Masahiro

  Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd.

• OKUMURA Hajime

  Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd.

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